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Assay Detail

CHEMBL4006785

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal His-tagged human recombinant FPPS expressed in Escherichia coli BL21 (DE3) using GPP and [3H]-IPP as substrate preincubated for 10 mins before substrate addition measured after 8 mins by liquid scintillation counting
28
Total Activities
28
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Farnesyl pyrophosphate synthase (CHEMBL1782)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pharmacophore Mapping of Thienopyrimidine-Based Monophosphonate (ThP-MP) Inhibitors of the Human Farnesyl Pyrophosphate Synthase.
Park J, Leung CY, Matralis AN, Lacbay CM, Tsakos M, Fernandez De Troconiz G, Berghuis AM, Tsantrizos YS.
J Med Chem (2017) 60 : 2119 -2134
PubMed 28208018 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 28 5.61 6.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4062596 1 6.07
CHEMBL1231307 1 6.07
CHEMBL4072263 1 6.06
CHEMBL4100337 1 6.00
CHEMBL4085285 1 6.00
CHEMBL4090564 1 5.96
CHEMBL4074061 1 5.85
CHEMBL4063665 1 5.82
CHEMBL4095113 1 5.80
CHEMBL3299149 1 5.80
CHEMBL4100741 1 5.80
CHEMBL4087424 1 5.64
CHEMBL4079542 1 5.58
CHEMBL4082744 1 5.55
CHEMBL4073585 1 5.52
CHEMBL3299051 1 5.48
CHEMBL4094213 1 5.43
CHEMBL4090167 1 5.40
CHEMBL4068738 1 5.34
CHEMBL4093024 1 5.34
CHEMBL4068147 1 5.32
CHEMBL3299047 1 5.30
CHEMBL4089846 1 5.08
CHEMBL4067698 1 5.06
CHEMBL4097575 1 5.00
CHEMBL4093973 1 -
CHEMBL4062145 1 -
CHEMBL4079020 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4062596 IC50 = 860.0 nM 6.07
CHEMBL1231307 IC50 = 850.0 nM 6.07
CHEMBL4072263 IC50 = 870.0 nM 6.06
CHEMBL4100337 IC50 = 1000.0 nM 6.00
CHEMBL4085285 IC50 = 1000.0 nM 6.00
CHEMBL4090564 IC50 = 1100.0 nM 5.96
CHEMBL4074061 IC50 = 1400.0 nM 5.85
CHEMBL4063665 IC50 = 1500.0 nM 5.82
CHEMBL4095113 IC50 = 1600.0 nM 5.80
CHEMBL3299149 IC50 = 1600.0 nM 5.80
CHEMBL4100741 IC50 = 1600.0 nM 5.80
CHEMBL4087424 IC50 = 2300.0 nM 5.64
CHEMBL4079542 IC50 = 2600.0 nM 5.58
CHEMBL4082744 IC50 = 2800.0 nM 5.55
CHEMBL4073585 IC50 = 3000.0 nM 5.52
CHEMBL3299051 IC50 = 3300.0 nM 5.48
CHEMBL4094213 IC50 = 3700.0 nM 5.43
CHEMBL4090167 IC50 = 4000.0 nM 5.40
CHEMBL4068738 IC50 = 4600.0 nM 5.34
CHEMBL4093024 IC50 = 4600.0 nM 5.34
CHEMBL4068147 IC50 = 4800.0 nM 5.32
CHEMBL3299047 IC50 = 5000.0 nM 5.30
CHEMBL4089846 IC50 = 8400.0 nM 5.08
CHEMBL4067698 IC50 = 8800.0 nM 5.06
CHEMBL4097575 IC50 = 10000.0 nM 5.00
CHEMBL4093973 IC50 >= 100000.0 nM -
CHEMBL4062145 IC50 >= 100000.0 nM -
CHEMBL4079020 IC50 > 100000.0 nM -