Assay Detail
Binding
CHEMBL4006962
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human N-terminal truncated GST-tagged DHFR (31 to 395 residues) expressed in Escherichia coli BL21(DE3) pyrD using DHO as substrate preincubated for 5 mins followed by substrate addition measured for 5 mins by DCIP oxidation based CoQ10 enzyme coupled assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design, synthesis, biological evaluation and X-ray structural studies of potent human dihydroorotate dehydrogenase inhibitors based on hydroxylated azole scaffolds.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 13 | 7.02 | 8.74 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL38434 | BREQUINAR | 2.0 | 1 | 8.74 |
| CHEMBL4105330 | — | — | 1 | 7.80 |
| CHEMBL4098306 | — | — | 1 | 7.75 |
| CHEMBL4090575 | — | — | 1 | 7.44 |
| CHEMBL4088968 | — | — | 1 | 7.39 |
| CHEMBL4081077 | — | — | 1 | 7.36 |
| CHEMBL4062046 | — | — | 1 | 7.35 |
| CHEMBL4071260 | — | — | 1 | 6.97 |
| CHEMBL1946659 | — | — | 1 | 6.54 |
| CHEMBL973 | TERIFLUNOMIDE | 4.0 | 1 | 6.41 |
| CHEMBL1946851 | — | — | 1 | 6.22 |
| CHEMBL4061824 | — | — | 1 | 6.07 |
| CHEMBL4089886 | — | — | 1 | 5.22 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL38434 | BREQUINAR | IC50 | = | 1.8 | nM | 8.74 |
| CHEMBL4105330 | — | IC50 | = | 16.0 | nM | 7.80 |
| CHEMBL4098306 | — | IC50 | = | 18.0 | nM | 7.75 |
| CHEMBL4090575 | — | IC50 | = | 36.0 | nM | 7.44 |
| CHEMBL4088968 | — | IC50 | = | 41.0 | nM | 7.39 |
| CHEMBL4081077 | — | IC50 | = | 44.0 | nM | 7.36 |
| CHEMBL4062046 | — | IC50 | = | 45.0 | nM | 7.35 |
| CHEMBL4071260 | — | IC50 | = | 108.0 | nM | 6.97 |
| CHEMBL1946659 | — | IC50 | = | 289.0 | nM | 6.54 |
| CHEMBL973 | TERIFLUNOMIDE | IC50 | = | 388.0 | nM | 6.41 |
| CHEMBL1946851 | — | IC50 | = | 599.0 | nM | 6.22 |
| CHEMBL4061824 | — | IC50 | = | 853.0 | nM | 6.07 |
| CHEMBL4089886 | — | IC50 | = | 6000.0 | nM | 5.22 |