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Assay Detail

CHEMBL4008023

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at Gal4-fused human MR LBD (734 to 985 residues) expressed in CHO-K1 cells after 5 to 6 hrs by luciferase reporter gene assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mineralocorticoid receptor (CHEMBL1994)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Modulating Mineralocorticoid Receptor with Non-steroidal Antagonists. New Opportunities for the Development of Potent and Selective Ligands without Off-Target Side Effects.
Martín-Martínez M, Pérez-Gordillo FL, Álvarez de la Rosa D, Rodríguez Y, Gerona-Navarro G, González-Muñiz R, Zhou MM.
J Med Chem (2017) 60 : 2629 -2650
PubMed 28051871 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.43 8.15

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4104632 1 8.15
CHEMBL2181927 FINERENONE 4.0 1 7.75
CHEMBL4094464 1 7.75
CHEMBL4066981 1 7.72
CHEMBL4099077 1 7.64
CHEMBL4096901 1 7.58
CHEMBL2181926 1 7.55
CHEMBL4102462 1 7.41
CHEMBL4068008 1 7.33
CHEMBL4089197 1 7.00
CHEMBL4084504 1 6.80
CHEMBL4081044 1 6.51

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4104632 IC50 = 7.0 nM 8.15
CHEMBL2181927 FINERENONE IC50 = 18.0 nM 7.75
CHEMBL4094464 IC50 = 18.0 nM 7.75
CHEMBL4066981 IC50 = 19.0 nM 7.72
CHEMBL4099077 IC50 = 23.0 nM 7.64
CHEMBL4096901 IC50 = 26.0 nM 7.58
CHEMBL2181926 IC50 = 28.0 nM 7.55
CHEMBL4102462 IC50 = 39.0 nM 7.41
CHEMBL4068008 IC50 = 47.0 nM 7.33
CHEMBL4089197 IC50 = 100.0 nM 7.00
CHEMBL4084504 IC50 = 160.0 nM 6.80
CHEMBL4081044 IC50 = 310.0 nM 6.51