Assay Detail
Binding
CHEMBL4008767
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human KDM1A/CoREST complex expressed in Escherichia coli using H3K4me1 peptide as substrate preincubated for 15 mins with enzyme followed by substrate addition measured after 12 mins by Amplex Ultra Red reagent based HRP enzyme coupled assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Publication
Thieno[3,2-b]pyrrole-5-carboxamides as New Reversible Inhibitors of Histone Lysine Demethylase KDM1A/LSD1. Part 1: High-Throughput Screening and Preliminary Exploration.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 3 | 4.39 | 4.57 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4066708 | — | — | 1 | 4.57 |
| CHEMBL3989843 | TRANYLCYPROMINE | 4.0 | 1 | 4.51 |
| CHEMBL4086085 | — | — | 1 | 4.08 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4066708 | — | IC50 | = | 27100.0 | nM | 4.57 |
| CHEMBL3989843 | TRANYLCYPROMINE | IC50 | = | 30600.0 | nM | 4.51 |
| CHEMBL4086085 | — | IC50 | = | 82400.0 | nM | 4.08 |