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Assay Detail

CHEMBL4009330

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of BTN3A1 in human Vgamma9Vdelta2 T cells assessed as induction of T effector cells mediated K562 cell lysis preincubated with K562 cells for 2 hrs followed by T effector cell addition measured after 4 hrs
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Butyrophilin subfamily 3 member A1 (CHEMBL4105758)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Phosphinophosphonates and Their Tris-pivaloyloxymethyl Prodrugs Reveal a Negatively Cooperative Butyrophilin Activation Mechanism.
Shippy RR, Lin X, Agabiti SS, Li J, Zangari BM, Foust BJ, Poe MM, Hsiao CC, Vinogradova O, Wiemer DF, Wiemer AJ.
J Med Chem (2017) 60 : 2373 -2382
PubMed 28218845 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.22 8.80

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL145233 1 8.80
CHEMBL4061531 1 6.55
CHEMBL4081059 1 5.14
CHEMBL4089656 1 4.39
CHEMBL4097373 1 -
CHEMBL4099094 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL145233 EC50 = 1.6 nM 8.80
CHEMBL4061531 EC50 = 280.0 nM 6.55
CHEMBL4081059 EC50 = 7300.0 nM 5.14
CHEMBL4089656 EC50 = 41000.0 nM 4.39
CHEMBL4097373 EC50 > 100000.0 nM -
CHEMBL4099094 EC50 > 100000.0 nM -