Assay Detail
Binding
CHEMBL4009341
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length recombinant human ALDH1A3 expressed in Escherichia coli BL21(DE3) assessed as reduction in dehydrogenase activity by measuring NAD(P)H level preincubated for 2 mins followed by addition of propionaldehyde as substrate in presence of NAD+ by spectrophotometric method
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Inhibition of the Aldehyde Dehydrogenase 1/2 Family by Psoralen and Coumarin Derivatives.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.93 | 6.77 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1349972 | — | — | 1 | 6.77 |
| CHEMBL4072941 | — | — | 1 | 6.57 |
| CHEMBL4099822 | — | — | 1 | 6.40 |
| CHEMBL4064364 | — | — | 1 | 5.96 |
| CHEMBL4072973 | — | — | 1 | 5.00 |
| CHEMBL1335126 | — | — | 1 | 4.85 |
| CHEMBL315348 | — | — | 1 | - |
| CHEMBL4090473 | — | — | 1 | - |
| CHEMBL486422 | DAIDZIN | — | 1 | - |
| CHEMBL4061563 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1349972 | — | IC50 | = | 170.0 | nM | 6.77 |
| CHEMBL4072941 | — | IC50 | = | 270.0 | nM | 6.57 |
| CHEMBL4099822 | — | IC50 | = | 400.0 | nM | 6.40 |
| CHEMBL4064364 | — | IC50 | = | 1100.0 | nM | 5.96 |
| CHEMBL4072973 | — | IC50 | = | 10000.0 | nM | 5.00 |
| CHEMBL1335126 | — | IC50 | = | 14000.0 | nM | 4.85 |
| CHEMBL315348 | — | IC50 | - | — | - | |
| CHEMBL4090473 | — | IC50 | - | — | - | |
| CHEMBL486422 | DAIDZIN | IC50 | > | 200000.0 | nM | - |
| CHEMBL4061563 | — | IC50 | - | — | - |