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Assay Detail

CHEMBL4011228

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human dopamine D2 receptor expressed in HEK293 cells coexpressing renilla luciferase-fused GalphaoA/GFP10-fused Ggamma2 assessed as inhibition of quinpirole-induced GalphaoA activation by BRET assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(2) dopamine receptor (CHEMBL217)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel Bivalent Ligands Based on the Sumanirole Pharmacophore Reveal Dopamine D2 Receptor (D2R) Biased Agonism.
Bonifazi A, Yano H, Ellenberger MP, Muller L, Kumar V, Zou MF, Cai NS, Guerrero AM, Woods AS, Shi L, Newman AH.
J Med Chem (2017) 60 : 2890 -2907
PubMed 28300398 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.04 7.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3797209 1 7.00
CHEMBL4076129 1 6.10
CHEMBL4068455 1 5.70
CHEMBL4093477 1 5.34
CHEMBL4097572 1 -
CHEMBL4077430 1 -
CHEMBL4100734 1 -
CHEMBL4100322 1 -
CHEMBL4085282 1 -
CHEMBL4095452 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3797209 IC50 = 98.9 nM 7.00
CHEMBL4076129 IC50 = 801.7 nM 6.10
CHEMBL4068455 IC50 = 2013.7 nM 5.70
CHEMBL4093477 IC50 = 4529.0 nM 5.34
CHEMBL4097572 IC50 > 5000.0 nM -
CHEMBL4077430 IC50 > 5000.0 nM -
CHEMBL4100734 IC50 > 5000.0 nM -
CHEMBL4100322 IC50 > 5000.0 nM -
CHEMBL4085282 IC50 > 5000.0 nM -
CHEMBL4095452 IC50 > 5000.0 nM -