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Assay Detail

CHEMBL4012328

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human Nav1.7 expressed in HEK293 cells at holding potential yielding 20 to 50% inactivation measured after 3 to 5 mins by patchXpress electrophysiology assay
37
Total Activities
32
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium channel protein type 9 subunit alpha (CHEMBL4296)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Sulfonamides as Selective NaV1.7 Inhibitors: Optimizing Potency, Pharmacokinetics, and Metabolic Properties to Obtain Atropisomeric Quinolinone (AM-0466) that Affords Robust in Vivo Activity.
Graceffa RF, Boezio AA, Able J, Altmann S, Berry LM, Boezio C, Butler JR, Chu-Moyer M, Cooke M, DiMauro EF, Dineen TA, Feric Bojic E, Foti RS, Fremeau RT, Guzman-Perez A, Gao H, Gunaydin H, Huang H, Huang L, Ilch C, Jarosh M, Kornecook T, Kreiman CR, La DS, Ligutti J, Milgram BC, Lin MJ, Marx IE, Nguyen HN, Peterson EA, Rescourio G, Roberts J, Schenkel L, Shimanovich R, Sparling BA, Stellwagen J, Taborn K, Vaida KR, Wang J, Yeoman J, Yu V, Zhu D, Moyer BD, Weiss MM.
J Med Chem (2017) 60 : 5990 -6017
PubMed 28324649 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 7.42 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3930653 1 8.52
CHEMBL3949474 1 8.30
CHEMBL3921690 1 8.15
CHEMBL3902872 1 8.15
CHEMBL3966606 1 8.14
CHEMBL3915688 2 8.10
CHEMBL3983367 1 8.05
CHEMBL3933527 1 8.00
CHEMBL3965976 1 8.00
CHEMBL3956563 1 8.00
CHEMBL3961163 1 7.96
CHEMBL3948535 1 7.96
CHEMBL4084372 1 7.92
CHEMBL3941727 1 7.92
CHEMBL3953660 1 7.82
CHEMBL3935378 1 7.77
CHEMBL3946521 1 7.77
CHEMBL3921528 1 7.75
CHEMBL3967168 1 7.72
CHEMBL3979498 1 7.68
CHEMBL3935302 1 7.66
CHEMBL3949600 1 7.64
CHEMBL3937744 1 7.58
CHEMBL3906185 1 7.57
CHEMBL4073429 3 7.57
CHEMBL3903048 1 7.16
CHEMBL3983724 1 6.92
CHEMBL4069051 1 6.80
CHEMBL3926166 1 6.68
CHEMBL3947470 3 6.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3930653 IC50 = 3.0 nM 8.52
CHEMBL3949474 IC50 = 5.0 nM 8.30
CHEMBL3902872 IC50 = 7.0 nM 8.15
CHEMBL3921690 IC50 = 7.0 nM 8.15
CHEMBL3966606 IC50 = 7.3 nM 8.14
CHEMBL3915688 IC50 = 8.0 nM 8.10
CHEMBL3983367 IC50 = 9.0 nM 8.05
CHEMBL3933527 IC50 = 10.0 nM 8.00
CHEMBL3956563 IC50 = 10.0 nM 8.00
CHEMBL3965976 IC50 = 10.0 nM 8.00
CHEMBL3948535 IC50 = 11.0 nM 7.96
CHEMBL3961163 IC50 = 11.0 nM 7.96
CHEMBL4084372 IC50 = 12.0 nM 7.92
CHEMBL3941727 IC50 = 12.0 nM 7.92
CHEMBL3953660 IC50 = 15.0 nM 7.82
CHEMBL3935378 IC50 = 17.0 nM 7.77
CHEMBL3946521 IC50 = 17.0 nM 7.77
CHEMBL3921528 IC50 = 18.0 nM 7.75
CHEMBL3967168 IC50 = 19.0 nM 7.72
CHEMBL3979498 IC50 = 21.0 nM 7.68
CHEMBL3935302 IC50 = 22.0 nM 7.66
CHEMBL3949600 IC50 = 23.0 nM 7.64
CHEMBL3915688 IC50 = 26.0 nM 7.58
CHEMBL3937744 IC50 = 26.0 nM 7.58
CHEMBL3906185 IC50 = 27.0 nM 7.57
CHEMBL4073429 IC50 = 27.0 nM 7.57
CHEMBL4073429 IC50 = 59.0 nM 7.23
CHEMBL3903048 IC50 = 69.0 nM 7.16
CHEMBL3983724 IC50 = 120.0 nM 6.92
CHEMBL4069051 IC50 = 160.0 nM 6.80
CHEMBL3926166 IC50 = 210.0 nM 6.68
CHEMBL3947470 IC50 = 240.0 nM 6.62
CHEMBL3928024 IC50 = 270.0 nM 6.57
CHEMBL3947470 IC50 = 500.0 nM 6.30
CHEMBL4071624 IC50 = 1800.0 nM 5.75
CHEMBL4073429 IC50 = 18000.0 nM 4.75
CHEMBL3947470 IC50 = 22000.0 nM 4.66