Assay Detail
Binding
CHEMBL4013057
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of acid ceramidase in human A375 cells using fluorogenic substrate Rbm-14-12 preincubated for 2 hrs followed by substrate addition measured after 3 hrs by fluorogenic assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Pharmacophore Identification and Scaffold Exploration to Discover Novel, Potent, and Chemically Stable Inhibitors of Acid Ceramidase in Melanoma Cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 5 | 6.42 | 7.12 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4100744 | — | — | 1 | 7.12 |
| CHEMBL460499 | CARMOFUR | 2.0 | 1 | 6.72 |
| CHEMBL4075846 | — | — | 1 | 6.30 |
| CHEMBL4094489 | — | — | 1 | 6.01 |
| CHEMBL4076865 | — | — | 1 | 5.97 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4100744 | — | IC50 | = | 75.6 | nM | 7.12 |
| CHEMBL460499 | CARMOFUR | IC50 | = | 190.8 | nM | 6.72 |
| CHEMBL4075846 | — | IC50 | = | 505.3 | nM | 6.30 |
| CHEMBL4094489 | — | IC50 | = | 966.9 | nM | 6.01 |
| CHEMBL4076865 | — | IC50 | = | 1061.5 | nM | 5.97 |