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Assay Detail

CHEMBL4014238

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human GPR34 expressed in HEK293 cells co-transfected with AP-TGFalpha/chimeric Galphaq/i1 assessed as induction of AP-TGFalpha release after 1 hr
20
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Probable G-protein coupled receptor 34 (CHEMBL3562165)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Probing the Hydrophobic Binding Pocket of G-Protein-Coupled Lysophosphatidylserine Receptor GPR34/LPS1 by Docking-Aided Structure-Activity Analysis.
Sayama M, Inoue A, Nakamura S, Jung S, Ikubo M, Otani Y, Uwamizu A, Kishi T, Makide K, Aoki J, Hirokawa T, Ohwada T.
J Med Chem (2017) 60 : 6384 -6399
PubMed 28715213 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 20 7.03 7.83

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4095241 2 7.83
CHEMBL4096239 2 7.57
CHEMBL4098603 2 7.39
CHEMBL4090880 2 7.11
CHEMBL4072692 2 7.05
CHEMBL3814092 2 6.82
CHEMBL4084094 2 6.75
CHEMBL4068969 2 6.64
CHEMBL1742484 2 6.61
CHEMBL4089962 2 6.53

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4095241 EC50 = 14.79 nM 7.83
CHEMBL4095241 EC50 = 15.0 nM 7.82
CHEMBL4096239 EC50 = 27.0 nM 7.57
CHEMBL4096239 EC50 = 26.92 nM 7.57
CHEMBL4098603 EC50 = 41.0 nM 7.39
CHEMBL4098603 EC50 = 40.74 nM 7.39
CHEMBL4090880 EC50 = 78.0 nM 7.11
CHEMBL4090880 EC50 = 77.62 nM 7.11
CHEMBL4072692 EC50 = 90.0 nM 7.05
CHEMBL4072692 EC50 = 95.5 nM 7.02
CHEMBL3814092 EC50 = 150.0 nM 6.82
CHEMBL3814092 EC50 = 151.36 nM 6.82
CHEMBL4084094 EC50 = 180.0 nM 6.75
CHEMBL4084094 EC50 = 181.97 nM 6.74
CHEMBL4068969 EC50 = 230.0 nM 6.64
CHEMBL4068969 EC50 = 229.09 nM 6.64
CHEMBL1742484 EC50 = 245.47 nM 6.61
CHEMBL1742484 EC50 = 250.0 nM 6.60
CHEMBL4089962 EC50 = 295.12 nM 6.53
CHEMBL4089962 EC50 = 300.0 nM 6.52