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Assay Detail

CHEMBL4014754

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of recombinant human glucokinase assessed as conversion of D-glucose to D-glucose-6-phosphate in presence of 2.5 mM glucose by G6PDH coupled spectrophotometric assay
14
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Hexokinase-4 (CHEMBL3820)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel, highly potent systemic glucokinase activators for the treatment of Type 2 Diabetes Mellitus.
Xu J, Lin S, Myers RW, Addona G, Berger JP, Campbell B, Chen HS, Chen Z, Eiermann GJ, Elowe NH, Farrer BT, Feng W, Fu Q, Kats-Kagan R, Kavana M, Malkani S, McMasters DR, Mitra K, Pachanski MJ, Tong X, Trujillo ME, Xu L, Zhang B, Zhang F, Zhang R, Parmee ER.
Bioorg Med Chem Lett (2017) 27 : 2069 -2073
PubMed 28284804 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 8.10 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4086782 1 8.70
CHEMBL4083339 2 8.62
CHEMBL4067028 2 8.43
CHEMBL4078915 2 8.35
CHEMBL4086771 2 8.35
CHEMBL4067037 1 8.32
CHEMBL4068048 2 8.32
CHEMBL4104680 1 8.09
CHEMBL4094511 1 7.62

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4086782 EC50 = 2.0 nM 8.70
CHEMBL4083339 EC50 = 2.4 nM 8.62
CHEMBL4067028 EC50 = 3.7 nM 8.43
CHEMBL4067028 EC50 = 4.2 nM 8.38
CHEMBL4078915 EC50 = 4.5 nM 8.35
CHEMBL4086771 EC50 = 4.5 nM 8.35
CHEMBL4067037 EC50 = 4.8 nM 8.32
CHEMBL4068048 EC50 = 4.8 nM 8.32
CHEMBL4086771 EC50 = 5.5 nM 8.26
CHEMBL4104680 EC50 = 8.2 nM 8.09
CHEMBL4094511 EC50 = 24.0 nM 7.62
CHEMBL4068048 EC50 = 31.0 nM 7.51
CHEMBL4078915 EC50 = 48.0 nM 7.32
CHEMBL4083339 EC50 = 86.0 nM 7.07