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Assay Detail

CHEMBL4015580

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HCV genotype 1b NS5B replicase expressed in Escherichia coli BL21(DE3) using polyA/biotinylated oligo(dT12) as template/primer in presence of [3H]UTP after 60 mins by SPA assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Hepatitis C virus subtype 1b
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of a Hepatitis C Virus NS5B Replicase Palm Site Allosteric Inhibitor (BMS-929075) Advanced to Phase 1 Clinical Studies.
Yeung KS, Beno BR, Parcella K, Bender JA, Grant-Young KA, Nickel A, Gunaga P, Anjanappa P, Bora RO, Selvakumar K, Rigat K, Wang YK, Liu M, Lemm J, Mosure K, Sheriff S, Wan C, Witmer M, Kish K, Hanumegowda U, Zhuo X, Shu YZ, Parker D, Haskell R, Ng A, Gao Q, Colston E, Raybon J, Grasela DM, Santone K, Gao M, Meanwell NA, Sinz M, Soars MG, Knipe JO, Roberts SB, Kadow JF.
J Med Chem (2017) 60 : 4369 -4385
PubMed 28430437 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 6.42 8.23

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4072654 1 8.23
CHEMBL4093370 1 8.10
CHEMBL4068920 1 7.58
CHEMBL4098643 1 7.39
CHEMBL4090003 1 7.04
CHEMBL4097730 1 6.92
CHEMBL4071621 1 6.72
CHEMBL4078065 1 6.44
CHEMBL4102335 1 6.19
CHEMBL4060248 1 6.11
CHEMBL4085948 1 5.96
CHEMBL4082149 1 5.66
CHEMBL4079698 1 5.44
CHEMBL4082472 1 5.24
CHEMBL3902813 1 5.08
CHEMBL4064415 1 4.68
CHEMBL4067131 1 -
CHEMBL4094593 1 -
CHEMBL4060584 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4072654 IC50 = 5.9 nM 8.23
CHEMBL4093370 IC50 = 8.0 nM 8.10
CHEMBL4068920 IC50 = 26.0 nM 7.58
CHEMBL4098643 IC50 = 41.0 nM 7.39
CHEMBL4090003 IC50 = 91.0 nM 7.04
CHEMBL4097730 IC50 = 120.0 nM 6.92
CHEMBL4071621 IC50 = 190.0 nM 6.72
CHEMBL4078065 IC50 = 360.0 nM 6.44
CHEMBL4102335 IC50 = 650.0 nM 6.19
CHEMBL4060248 IC50 = 780.0 nM 6.11
CHEMBL4085948 IC50 = 1100.0 nM 5.96
CHEMBL4082149 IC50 = 2200.0 nM 5.66
CHEMBL4079698 IC50 = 3600.0 nM 5.44
CHEMBL4082472 IC50 = 5700.0 nM 5.24
CHEMBL3902813 IC50 = 8400.0 nM 5.08
CHEMBL4064415 IC50 = 21000.0 nM 4.68
CHEMBL4067131 IC50 > 50000.0 nM -
CHEMBL4094593 IC50 > 50000.0 nM -
CHEMBL4060584 IC50 > 50000.0 nM -