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Assay Detail

CHEMBL4015697

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of ABCB1 in human A2780adr cells assessed as reduction in calcein AM levels preincubated for 30 mins followed by calcein AM addition measured immediately at 60 sec time interval for 60 mins by fluorescence assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

ATP-dependent translocase ABCB1 (CHEMBL4302)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

4-Anilino-2-pyridylquinazolines and -pyrimidines as Highly Potent and Nontoxic Inhibitors of Breast Cancer Resistance Protein (ABCG2).
Krapf MK, Gallus J, Wiese M.
J Med Chem (2017) 60 : 4474 -4495
PubMed 28471656 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.50 6.48

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4099533 1 6.48
CHEMBL160 CYCLOSPORINE 4.0 1 5.92
CHEMBL4082355 1 5.68
CHEMBL4060577 1 5.56
CHEMBL4100665 1 5.51
CHEMBL3770841 1 5.43
CHEMBL4071162 1 5.32
CHEMBL4090505 1 5.26
CHEMBL4082677 1 5.20
CHEMBL1445052 1 5.20
CHEMBL4098216 1 4.90

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4099533 IC50 = 334.0 nM 6.48
CHEMBL160 CYCLOSPORINE IC50 = 1210.0 nM 5.92
CHEMBL4082355 IC50 = 2080.0 nM 5.68
CHEMBL4060577 IC50 = 2780.0 nM 5.56
CHEMBL4100665 IC50 = 3110.0 nM 5.51
CHEMBL3770841 IC50 = 3670.0 nM 5.43
CHEMBL4071162 IC50 = 4780.0 nM 5.32
CHEMBL4090505 IC50 = 5430.0 nM 5.26
CHEMBL4082677 IC50 = 6300.0 nM 5.20
CHEMBL1445052 IC50 = 6370.0 nM 5.20
CHEMBL4098216 IC50 = 12700.0 nM 4.90