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Assay Detail

CHEMBL4016546

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of PI3Kdelta in human whole blood assessed as reduction in anti-CD3/anti-CD28-mediated IFNgamma production preincubated for 1 hr followed by anti-CD3/anti-CD28 activation measured after overnight incubation by AlphaLisa assay
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Identification of a Potent, Selective, and Efficacious Phosphatidylinositol 3-Kinase δ (PI3Kδ) Inhibitor for the Treatment of Immunological Disorders.
Liu Q, Shi Q, Marcoux D, Batt DG, Cornelius L, Qin LY, Ruan Z, Neels J, Beaudoin-Bertrand M, Srivastava AS, Li L, Cherney RJ, Gong H, Watterson SH, Weigelt C, Gillooly KM, McIntyre KW, Xie JH, Obermeier MT, Fura A, Sleczka B, Stefanski K, Fancher RM, Padmanabhan S, Rp T, Kundu I, Rajareddy K, Smith R, Hennan JK, Xing D, Fan J, Levesque PC, Ruan Q, Pitt S, Zhang R, Pedicord D, Pan J, Yarde M, Lu H, Lippy J, Goldstine C, Skala S, Rampulla RA, Mathur A, Gupta A, Arunachalam PN, Sack JS, Muckelbauer JK, Cvijic ME, Salter-Cid LM, Bhide RS, Poss MA, Hynes J, Carter PH, Macor JE, Ruepp S, Schieven GL, Tino JA.
J Med Chem (2017) 60 : 5193 -5208
PubMed 28541707 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.03 7.55

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4074315 1 7.55
CHEMBL4064666 1 7.46
CHEMBL4092344 1 7.44
CHEMBL4100099 1 7.25
CHEMBL4059848 1 7.15
CHEMBL4094814 1 7.09
CHEMBL4078237 1 7.01
CHEMBL4070029 1 6.92
CHEMBL4102552 1 6.92
CHEMBL4073255 1 6.85
CHEMBL4097222 1 6.52
CHEMBL4084604 1 6.25

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4074315 IC50 = 28.0 nM 7.55
CHEMBL4064666 IC50 = 35.0 nM 7.46
CHEMBL4092344 IC50 = 36.0 nM 7.44
CHEMBL4100099 IC50 = 56.0 nM 7.25
CHEMBL4059848 IC50 = 71.0 nM 7.15
CHEMBL4094814 IC50 = 82.0 nM 7.09
CHEMBL4078237 IC50 = 98.0 nM 7.01
CHEMBL4070029 IC50 = 120.0 nM 6.92
CHEMBL4102552 IC50 = 120.0 nM 6.92
CHEMBL4073255 IC50 = 140.0 nM 6.85
CHEMBL4097222 IC50 = 300.0 nM 6.52
CHEMBL4084604 IC50 = 560.0 nM 6.25