Assay Detail
Binding
CHEMBL4016699
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of full length human C-terminal His6-tagged CDK2/N-terminal GST-tagged CyclinE expressed in baculovirus infected sf21 cells after 10 mins in presence of [gamma32P]ATP by beta counting method
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 7 — Homologous single protein target |
| Curated By | Autocuration |
Target
Cyclin-dependent kinase 2/cyclin E1 (CHEMBL1907605) ↗| Type | PROTEIN COMPLEX |
| Organism | Homo sapiens |
Publication
Identification of a Water-Soluble Indirubin Derivative as Potent Inhibitor of Insulin-like Growth Factor 1 Receptor through Structural Modification of the Parent Natural Molecule.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 6.46 | 7.37 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4065365 | — | — | 1 | 7.37 |
| CHEMBL1830064 | — | — | 1 | 7.27 |
| CHEMBL4085289 | — | — | 1 | 7.05 |
| CHEMBL1276317 | — | — | 1 | 6.68 |
| CHEMBL1802727 | — | — | 1 | 6.64 |
| CHEMBL14762 | SELICICLIB | 2.0 | 1 | 6.21 |
| CHEMBL4100748 | — | — | 1 | 6.04 |
| CHEMBL4100916 | — | — | 1 | 5.77 |
| CHEMBL1276127 | INDIRUBIN | 2.0 | 1 | 5.12 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4065365 | — | IC50 | = | 43.0 | nM | 7.37 |
| CHEMBL1830064 | — | IC50 | = | 54.0 | nM | 7.27 |
| CHEMBL4085289 | — | IC50 | = | 90.0 | nM | 7.05 |
| CHEMBL1276317 | — | IC50 | = | 210.0 | nM | 6.68 |
| CHEMBL1802727 | — | IC50 | = | 230.0 | nM | 6.64 |
| CHEMBL14762 | SELICICLIB | IC50 | = | 620.0 | nM | 6.21 |
| CHEMBL4100748 | — | IC50 | = | 920.0 | nM | 6.04 |
| CHEMBL4100916 | — | IC50 | = | 1700.0 | nM | 5.77 |
| CHEMBL1276127 | INDIRUBIN | IC50 | = | 7500.0 | nM | 5.12 |