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Assay Detail

CHEMBL4017315

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against HCV genotype 1b harboring wild type NS5B infected in human HuH7 cells assessed as inhibition of viral RNA replication after 5 days by RT-PCR method
32
Total Activities
16
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Hepacivirus hominis
Confidence 1 — Organism
Curated By Autocuration

Target

Hepatitis C virus (CHEMBL379)
Type ORGANISM
Organism Hepacivirus hominis

Publication

2'-Chloro,2'-fluoro Ribonucleotide Prodrugs with Potent Pan-genotypic Activity against Hepatitis C Virus Replication in Culture.
Zhou S, Mahmoud S, Liu P, Zhou L, Ehteshami M, Bassit L, Tao S, Domaoal RA, Sari O, Schutter C, Amiralaei S, Khalil A, Ollinger Russell O, McBrayer T, Whitaker T, Abou-Taleb N, Amblard F, Coats SJ, Schinazi RF.
J Med Chem (2017) 60 : 5424 -5437
PubMed 28595015 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.44 7.70
EC90 16 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4081718 2 7.70
CHEMBL1259059 SOFOSBUVIR 4.0 2 7.05
CHEMBL4065765 2 7.00
CHEMBL4093378 2 6.52
CHEMBL4095814 2 6.52
CHEMBL4074383 2 6.22
CHEMBL438605 2 5.77
CHEMBL4077782 2 4.72
CHEMBL4099733 2 -
CHEMBL4101127 2 -
CHEMBL4085639 2 -
CHEMBL4103568 2 -
CHEMBL4082161 2 -
CHEMBL4100162 2 -
CHEMBL4069879 2 -
CHEMBL4090878 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4081718 EC50 = 20.0 nM 7.70
CHEMBL1259059 SOFOSBUVIR EC50 = 90.0 nM 7.05
CHEMBL4065765 EC50 = 100.0 nM 7.00
CHEMBL4093378 EC50 = 300.0 nM 6.52
CHEMBL4095814 EC50 = 300.0 nM 6.52
CHEMBL4074383 EC50 = 600.0 nM 6.22
CHEMBL438605 EC50 = 1700.0 nM 5.77
CHEMBL4077782 EC50 = 19000.0 nM 4.72
CHEMBL4101127 EC90 > 10.0 uM -
CHEMBL4101127 EC50 > 10000.0 nM -
CHEMBL4065765 EC90 = 0.3 uM -
CHEMBL4093378 EC90 = 0.9 uM -
CHEMBL4081718 EC90 = 0.07 uM -
CHEMBL4099733 EC90 > 100.0 uM -
CHEMBL4090878 EC50 > 10000.0 nM -
CHEMBL4069879 EC50 > 10000.0 nM -
CHEMBL4100162 EC50 > 10000.0 nM -
CHEMBL4082161 EC50 > 10000.0 nM -
CHEMBL4103568 EC50 > 10000.0 nM -
CHEMBL4085639 EC50 > 10000.0 nM -
CHEMBL4099733 EC50 > 100000.0 nM -
CHEMBL438605 EC90 = 6.5 uM -
CHEMBL4090878 EC90 > 10.0 uM -
CHEMBL4069879 EC90 > 10.0 uM -
CHEMBL4100162 EC90 > 10.0 uM -
CHEMBL4082161 EC90 > 10.0 uM -
CHEMBL4103568 EC90 > 10.0 uM -
CHEMBL4085639 EC90 > 10.0 uM -
CHEMBL4095814 EC90 = 0.8 uM -
CHEMBL4077782 EC90 = 33.0 uM -
CHEMBL1259059 SOFOSBUVIR EC90 = 0.3 uM -
CHEMBL4074383 EC90 = 2.1 uM -