Assay Detail

CHEMBL4017334

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Functional

Antiviral activity against HCV genotype 1b harboring NS5B S282T mutant infected in human HuH7 cells assessed as inhibition of viral RNA replication after 4 days by luciferase reporter gene assay

ChEMBL ↗

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Target

Hepatitis C virus (CHEMBL379) ↗

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Primary target Hepatitis C virus

CHEMBL379

Anchored from Assay target.

Protein identity Hepatitis C virus

Disease frame Disease evidence pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Hepacivirus hominis
Confidence	1 — Organism
Curated By	Autocuration

Target

Hepatitis C virus (CHEMBL379) ↗

Type	ORGANISM
Organism	Hepacivirus hominis

Publication

2'-Chloro,2'-fluoro Ribonucleotide Prodrugs with Potent Pan-genotypic Activity against Hepatitis C Virus Replication in Culture.

Zhou S, Mahmoud S, Liu P, Zhou L, Ehteshami M, Bassit L, Tao S, Domaoal RA, Sari O, Schutter C, Amiralaei S, Khalil A, Ollinger Russell O, McBrayer T, Whitaker T, Abou-Taleb N, Amblard F, Coats SJ, Schinazi RF.
J Med Chem (2017) 60 : 5424 -5437
PubMed 28595015 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
EC50	2	6.78	7.16
EC90	2	-	-

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL4081718	—	—	2	7.16
CHEMBL1259059	SOFOSBUVIR	4.0	2	6.40

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL4081718	—	EC50	=	70.0	nM	7.16
CHEMBL1259059	SOFOSBUVIR	EC50	=	400.0	nM	6.40
CHEMBL4081718	—	EC90	=	0.2	uM	-
CHEMBL1259059	SOFOSBUVIR	EC90	=	1.0	uM	-