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Assay Detail

CHEMBL4019028

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Serratia marcescens IMP1 expressed in Escherichia coli BL21(DE3) using chromacef as substrate preincubated for 10 mins followed by substrate addition measured after 20 mins by HTS assay
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Serratia marcescens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metallo-beta-lactamase type 2 (CHEMBL4105964)
Type SINGLE PROTEIN
Organism Serratia marcescens

Publication

Dipicolinic Acid Derivatives as Inhibitors of New Delhi Metallo-β-lactamase-1.
Chen AY, Thomas PW, Stewart AC, Bergstrom A, Cheng Z, Miller C, Bethel CR, Marshall SH, Credille CV, Riley CL, Page RC, Bonomo RA, Crowder MW, Tierney DL, Fast W, Cohen SM.
J Med Chem (2017) 60 : 7267 -7283
PubMed 28809565 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 6.07 6.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4068259 1 6.62
CHEMBL284104 DIPICOLINIC ACID 1 5.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4068259 IC50 = 240.0 nM 6.62
CHEMBL284104 DIPICOLINIC ACID IC50 = 3030.0 nM 5.52