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Assay Detail

CHEMBL4019299

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mTOR in human A2058 cells assessed as reduction in phosphorylation of S6 at ser235/236 residues after 1 hr by in-cell Western method
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL2842)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

5-(4,6-Dimorpholino-1,3,5-triazin-2-yl)-4-(trifluoromethyl)pyridin-2-amine (PQR309), a Potent, Brain-Penetrant, Orally Bioavailable, Pan-Class I PI3K/mTOR Inhibitor as Clinical Candidate in Oncology.
Beaufils F, Cmiljanovic N, Cmiljanovic V, Bohnacker T, Melone A, Marone R, Jackson E, Zhang X, Sele A, Borsari C, Mestan J, Hebeisen P, Hillmann P, Giese B, Zvelebil M, Fabbro D, Williams RL, Rageot D, Wymann MP.
J Med Chem (2017) 60 : 7524 -7538
PubMed 28829592 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.10 6.69

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4084907 BIMIRALISIB 2.0 1 6.69
CHEMBL4102855 1 6.51
CHEMBL4097282 1 6.38
CHEMBL4068426 1 6.38
CHEMBL4081904 1 6.14
CHEMBL4079258 1 6.09
CHEMBL4092165 1 6.08
CHEMBL4071150 1 6.03
CHEMBL4098203 1 5.96
CHEMBL4089567 1 5.89
CHEMBL4071370 1 5.79
CHEMBL4074098 1 5.25
CHEMBL4062723 1 -
CHEMBL4100862 1 -
CHEMBL4099904 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4084907 BIMIRALISIB IC50 = 205.0 nM 6.69
CHEMBL4102855 IC50 = 312.0 nM 6.51
CHEMBL4097282 IC50 = 415.0 nM 6.38
CHEMBL4068426 IC50 = 415.0 nM 6.38
CHEMBL4081904 IC50 = 729.0 nM 6.14
CHEMBL4079258 IC50 = 817.0 nM 6.09
CHEMBL4092165 IC50 = 831.0 nM 6.08
CHEMBL4071150 IC50 = 939.0 nM 6.03
CHEMBL4098203 IC50 = 1105.0 nM 5.96
CHEMBL4089567 IC50 = 1282.0 nM 5.89
CHEMBL4071370 IC50 = 1638.0 nM 5.79
CHEMBL4074098 IC50 = 5588.0 nM 5.25
CHEMBL4062723 IC50 - -
CHEMBL4100862 IC50 = 1100000.0 nM -
CHEMBL4099904 IC50 = 1.7 10'7nM -