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Assay Detail

CHEMBL4019300

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human N-terminal His6-tagged PI3K p110alpha/p85alpha expressed in baculovirus expression system after 1 hr using AlexaFluor647-labeled kinase tracer 314 by TR-FRET assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

PI3-kinase p110-alpha/p85-alpha (CHEMBL2111367)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

5-(4,6-Dimorpholino-1,3,5-triazin-2-yl)-4-(trifluoromethyl)pyridin-2-amine (PQR309), a Potent, Brain-Penetrant, Orally Bioavailable, Pan-Class I PI3K/mTOR Inhibitor as Clinical Candidate in Oncology.
Beaufils F, Cmiljanovic N, Cmiljanovic V, Bohnacker T, Melone A, Marone R, Jackson E, Zhang X, Sele A, Borsari C, Mestan J, Hebeisen P, Hillmann P, Giese B, Zvelebil M, Fabbro D, Williams RL, Rageot D, Wymann MP.
J Med Chem (2017) 60 : 7524 -7538
PubMed 28829592 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 6.71 8.09

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4102855 1 8.09
CHEMBL4081904 1 8.01
CHEMBL4092165 1 7.89
CHEMBL4084907 BIMIRALISIB 2.0 1 7.77
CHEMBL4097282 1 7.34
CHEMBL4071150 1 7.21
CHEMBL4068426 1 6.91
CHEMBL4074098 1 6.16
CHEMBL4079258 1 6.04
CHEMBL4098203 1 5.92
CHEMBL4089567 1 5.82
CHEMBL4099904 1 5.64
CHEMBL4100862 1 4.48
CHEMBL4062723 1 -
CHEMBL4071370 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4102855 Ki = 8.1 nM 8.09
CHEMBL4081904 Ki = 9.7 nM 8.01
CHEMBL4092165 Ki = 13.0 nM 7.89
CHEMBL4084907 BIMIRALISIB Ki = 17.0 nM 7.77
CHEMBL4097282 Ki = 46.0 nM 7.34
CHEMBL4071150 Ki = 61.0 nM 7.21
CHEMBL4068426 Ki = 123.0 nM 6.91
CHEMBL4074098 Ki = 689.0 nM 6.16
CHEMBL4079258 Ki = 911.0 nM 6.04
CHEMBL4098203 Ki = 1205.0 nM 5.92
CHEMBL4089567 Ki = 1513.0 nM 5.82
CHEMBL4099904 Ki = 2299.0 nM 5.64
CHEMBL4100862 Ki = 32833.0 nM 4.48
CHEMBL4062723 Ki - -
CHEMBL4071370 Ki = 4300000.0 nM -