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Assay Detail

CHEMBL4021376

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant full length C-terminal His/FLAG-tagged human HDAC1 expressed in baculovirus infected Sf9 insect cells using BPS HDAC substrate 3 after 30 mins by fluorescence assay
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histone deacetylase 1 (CHEMBL325)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of largazole zinc-binding group analogs.
Kim B, Ratnayake R, Lee H, Shi G, Zeller SL, Li C, Luesch H, Hong J.
Bioorg Med Chem (2017) 25 : 3077 -3086
PubMed 28416100 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 8.13 9.40

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3593247 1 9.40
CHEMBL4099942 1 7.58
CHEMBL4078721 1 7.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3593247 IC50 = 0.4 nM 9.40
CHEMBL4099942 IC50 = 26.0 nM 7.58
CHEMBL4078721 IC50 = 40.0 nM 7.40