Assay Detail
Binding
CHEMBL4022774
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of AICARFT in human NCI-H460 cells assessed as increase in ZMP levels using low folate media after 16 hrs by LC-MS method
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Bifunctional purine biosynthesis protein ATIC (CHEMBL2518) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
Discovery of N-(6-Fluoro-1-oxo-1,2-dihydroisoquinolin-7-yl)-5-[(3R)-3-hydroxypyrrolidin-1-yl]thiophene-2-sulfonamide (LSN 3213128), a Potent and Selective Nonclassical Antifolate Aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase (AICARFT) Inhibitor Effective at Tumor Suppression in a Cancer Xenograft Model.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 18 | 7.49 | 8.40 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4100363 | — | — | 1 | 8.40 |
| CHEMBL4081385 | — | — | 1 | 8.10 |
| CHEMBL4063104 | — | — | 1 | 8.10 |
| CHEMBL4083899 | — | — | 1 | 8.00 |
| CHEMBL4099409 | — | — | 1 | 7.77 |
| CHEMBL4079085 | — | — | 1 | 7.75 |
| CHEMBL4076500 | — | — | 1 | 7.68 |
| CHEMBL4074469 | — | — | 1 | 7.60 |
| CHEMBL4091668 | — | — | 1 | 7.57 |
| CHEMBL4070790 | — | — | 1 | 7.51 |
| CHEMBL4075503 | — | — | 1 | 7.21 |
| CHEMBL4101204 | — | — | 1 | 7.17 |
| CHEMBL4092503 | — | — | 1 | 6.25 |
| CHEMBL4084757 | — | — | 1 | 5.74 |
| CHEMBL4101760 | — | — | 1 | - |
| CHEMBL4077027 | — | — | 1 | - |
| CHEMBL4082237 | — | — | 1 | - |
| CHEMBL4100255 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4100363 | — | EC50 | = | 4.0 | nM | 8.40 |
| CHEMBL4081385 | — | EC50 | = | 7.9 | nM | 8.10 |
| CHEMBL4063104 | — | EC50 | = | 8.0 | nM | 8.10 |
| CHEMBL4083899 | — | EC50 | = | 10.0 | nM | 8.00 |
| CHEMBL4099409 | — | EC50 | = | 17.0 | nM | 7.77 |
| CHEMBL4079085 | — | EC50 | = | 18.0 | nM | 7.75 |
| CHEMBL4076500 | — | EC50 | = | 21.0 | nM | 7.68 |
| CHEMBL4074469 | — | EC50 | = | 25.0 | nM | 7.60 |
| CHEMBL4091668 | — | EC50 | = | 27.0 | nM | 7.57 |
| CHEMBL4070790 | — | EC50 | = | 31.0 | nM | 7.51 |
| CHEMBL4075503 | — | EC50 | = | 62.0 | nM | 7.21 |
| CHEMBL4101204 | — | EC50 | = | 68.0 | nM | 7.17 |
| CHEMBL4092503 | — | EC50 | = | 567.0 | nM | 6.25 |
| CHEMBL4084757 | — | EC50 | = | 1811.0 | nM | 5.74 |
| CHEMBL4101760 | — | EC50 | - | — | - | |
| CHEMBL4077027 | — | EC50 | - | — | - | |
| CHEMBL4082237 | — | EC50 | - | — | - | |
| CHEMBL4100255 | — | EC50 | - | — | - |