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Assay Detail

CHEMBL4025647

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human NCI-H460 cells pretreated for 24 hrs under hypoxic condition followed by compound washout measured after 72 hrs by MTT assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H460
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H460 (CHEMBL396)
Type CELL-LINE
Organism Homo sapiens

Publication

Synthesis and biological evaluation of hypoxia-activated prodrugs of SN-38.
Jin C, Zhang Q, Lu W.
Eur J Med Chem (2017) 132 : 135 -141
PubMed 28350997 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 6.06 7.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL837 7-ETHYL-10-HYDROXYCAMPTOTHECIN 2.0 1 7.22
CHEMBL4079921 1 6.85
CHEMBL4059463 1 5.12
CHEMBL260046 EVOFOSFAMIDE 3.0 1 5.04

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL837 7-ETHYL-10-HYDROXYCAMPTOTHECIN IC50 = 60.0 nM 7.22
CHEMBL4079921 IC50 = 140.0 nM 6.85
CHEMBL4059463 IC50 = 7560.0 nM 5.12
CHEMBL260046 EVOFOSFAMIDE IC50 = 9080.0 nM 5.04