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Assay Detail

CHEMBL4026560

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of D5D in human HepG2 cells assessed as [14C]AA formation from [14C]DGLA preincubated for 30 mins followed by [14C]eicosatrienoic acid addition measured after 3 hrs by TLC analysis
20
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acyl-CoA (8-3)-desaturase (CHEMBL5840)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 3,5-Diphenyl-4-methyl-1,3-oxazolidin-2-ones as Novel, Potent, and Orally Available Δ-5 Desaturase (D5D) Inhibitors.
Fujimoto J, Okamoto R, Noguchi N, Hara R, Masada S, Kawamoto T, Nagase H, Tamura YO, Imanishi M, Takagahara S, Kubo K, Tohyama K, Iida K, Andou T, Miyahisa I, Matsui J, Hayashi R, Maekawa T, Matsunaga N.
J Med Chem (2017) 60 : 8963 -8981
PubMed 29023121 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 7.59 9.28

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4084502 1 9.28
CHEMBL4064555 1 8.82
CHEMBL4102458 1 8.43
CHEMBL4060775 1 8.39
CHEMBL4070383 2 8.06
CHEMBL4090490 1 7.75
CHEMBL4104260 1 7.26
CHEMBL4066595 1 7.22
CHEMBL4069465 1 6.82
CHEMBL4087912 1 6.77
CHEMBL4080025 1 6.77
CHEMBL4085437 1 6.72
CHEMBL4063561 1 5.96
CHEMBL4084463 1 -
CHEMBL4062788 1 -
CHEMBL4073142 1 -
CHEMBL4069792 1 -
CHEMBL4091399 1 -
CHEMBL4072166 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4084502 IC50 = 0.52 nM 9.28
CHEMBL4064555 IC50 = 1.5 nM 8.82
CHEMBL4102458 IC50 = 3.7 nM 8.43
CHEMBL4060775 IC50 = 4.1 nM 8.39
CHEMBL4070383 IC50 = 8.7 nM 8.06
CHEMBL4070383 IC50 = 8.9 nM 8.05
CHEMBL4090490 IC50 = 18.0 nM 7.75
CHEMBL4104260 IC50 = 55.0 nM 7.26
CHEMBL4066595 IC50 = 60.0 nM 7.22
CHEMBL4069465 IC50 = 150.0 nM 6.82
CHEMBL4087912 IC50 = 170.0 nM 6.77
CHEMBL4080025 IC50 = 170.0 nM 6.77
CHEMBL4085437 IC50 = 190.0 nM 6.72
CHEMBL4063561 IC50 = 1100.0 nM 5.96
CHEMBL4084463 IC50 - -
CHEMBL4062788 IC50 - -
CHEMBL4073142 IC50 - -
CHEMBL4069792 IC50 - -
CHEMBL4091399 IC50 - -
CHEMBL4072166 IC50 - -