Assay Detail
Binding
CHEMBL4026799
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human ALK F1174L mutant (1093 to 1411 residues) expressed in baculovirus expression system using 5'FAM-KKSRGDYMTMQIG-CONH as substrate preincubated for 15 mins followed by addition of ATP measured after 1 hr by microfluidic mobility shift assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Identification of 4-Phenoxyquinoline Based Inhibitors for L1196M Mutant of Anaplastic Lymphoma Kinase by Structure-Based Design.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 7.62 | 8.80 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL388978 | STAUROSPORINE | — | 1 | 8.80 |
| CHEMBL4065208 | — | — | 1 | 8.03 |
| CHEMBL4091441 | — | — | 1 | 6.85 |
| CHEMBL4087615 | — | — | 1 | 6.81 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL388978 | STAUROSPORINE | IC50 | = | 1.6 | nM | 8.80 |
| CHEMBL4065208 | — | IC50 | = | 9.3 | nM | 8.03 |
| CHEMBL4091441 | — | IC50 | = | 141.0 | nM | 6.85 |
| CHEMBL4087615 | — | IC50 | = | 154.0 | nM | 6.81 |