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Assay Detail

CHEMBL4028145

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human GAL4-DBD fused MR ligand binding domain transfected in human Huh7 cells co-expressing GAL4-RE-Luc assessed as reduction in aldosterone-induced luciferase activity after 16 hrs in presence of serum by luciferase reporter gene assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mineralocorticoid receptor (CHEMBL1994)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of Morpholino-2H-pyrido[3,2-b][1,4]oxazin-3(4H)-ones as Nonsteroidal Mineralocorticoid Antagonists.
Piotrowski DW, Futatsugi K, Casimiro-Garcia A, Wei L, Sammons MF, Herr M, Jiao W, Lavergne SY, Coffey SB, Wright SW, Song K, Loria PM, Banker ME, Petersen DN, Bauman J.
J Med Chem (2018) 61 : 1086 -1097
PubMed 29300474 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.54 7.62

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4097160 1 7.62
CHEMBL4086109 1 7.48
CHEMBL2181928 1 7.36
CHEMBL4064593 1 7.26
CHEMBL4100538 1 7.01
CHEMBL4078214 1 6.98
CHEMBL4098088 1 6.60
CHEMBL4079143 1 6.58
CHEMBL4059632 1 6.45
CHEMBL4082524 1 6.39
CHEMBL4062345 1 6.23
CHEMBL4062371 1 5.92
CHEMBL4079083 1 5.71
CHEMBL4071008 1 5.52
CHEMBL4081584 1 5.00

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4097160 IC50 = 24.0 nM 7.62
CHEMBL4086109 IC50 = 33.0 nM 7.48
CHEMBL2181928 IC50 = 44.0 nM 7.36
CHEMBL4064593 IC50 = 55.0 nM 7.26
CHEMBL4100538 IC50 = 98.0 nM 7.01
CHEMBL4078214 IC50 = 105.0 nM 6.98
CHEMBL4098088 IC50 = 252.0 nM 6.60
CHEMBL4079143 IC50 = 266.0 nM 6.58
CHEMBL4059632 IC50 = 358.0 nM 6.45
CHEMBL4082524 IC50 = 407.0 nM 6.39
CHEMBL4062345 IC50 = 583.0 nM 6.23
CHEMBL4062371 IC50 = 1217.0 nM 5.92
CHEMBL4079083 IC50 = 1969.0 nM 5.71
CHEMBL4071008 IC50 = 2992.0 nM 5.52
CHEMBL4081584 IC50 = 10000.0 nM 5.00