Assay Detail
Binding
CHEMBL4031306
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Binding affinity to N-terminal His tagged human recombinant JAK2 (1014 to 1132 residues) expressed in Escherichia coli after 60 to 90 mins by LC/MS analysis
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Design and Synthesis of Ligand Efficient Dual Inhibitors of Janus Kinase (JAK) and Histone Deacetylase (HDAC) Based on Ruxolitinib and Vorinostat.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Kd | 10 | 5.35 | 6.00 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL4098975 | — | — | 1 | 6.00 |
| CHEMBL4084049 | — | — | 1 | 5.70 |
| CHEMBL4091233 | — | — | 1 | 5.70 |
| CHEMBL4069283 | — | — | 1 | 5.70 |
| CHEMBL4060201 | — | — | 1 | 5.30 |
| CHEMBL4089113 | — | — | 1 | 5.30 |
| CHEMBL4060602 | — | — | 1 | 5.22 |
| CHEMBL4088824 | — | — | 1 | 5.00 |
| CHEMBL1630770 | — | — | 1 | 4.26 |
| CHEMBL4070306 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL4098975 | — | Kd | = | 1000.0 | nM | 6.00 |
| CHEMBL4084049 | — | Kd | = | 2000.0 | nM | 5.70 |
| CHEMBL4091233 | — | Kd | = | 2000.0 | nM | 5.70 |
| CHEMBL4069283 | — | Kd | = | 2000.0 | nM | 5.70 |
| CHEMBL4060201 | — | Kd | = | 5000.0 | nM | 5.30 |
| CHEMBL4089113 | — | Kd | = | 5000.0 | nM | 5.30 |
| CHEMBL4060602 | — | Kd | = | 6000.0 | nM | 5.22 |
| CHEMBL4088824 | — | Kd | = | 10000.0 | nM | 5.00 |
| CHEMBL1630770 | — | Kd | = | 55000.0 | nM | 4.26 |
| CHEMBL4070306 | — | Kd | < | 1000.0 | nM | - |