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Assay Detail

CHEMBL4033007

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of JAK2 in EPO-stimulated human TF1 cells expressing stably integrated beta-lactamase reporter gene under control of STAT5 response elements in interferon regulatory factor 1 gene promoter by fluorescence assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Tyrosine-protein kinase JAK2 (CHEMBL2971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The Discovery of 3-((4-Chloro-3-methoxyphenyl)amino)-1-((3R,4S)-4-cyanotetrahydro-2H-pyran-3-yl)-1H-pyrazole-4-carboxamide, a Highly Ligand Efficient and Efficacious Janus Kinase 1 Selective Inhibitor with Favorable Pharmacokinetic Properties.
Siu T, Brubaker J, Fuller P, Torres L, Zeng H, Close J, Mampreian DM, Shi F, Liu D, Fradera X, Johnson K, Bays N, Kadic E, He F, Goldenblatt P, Shaffer L, Patel SB, Lesburg CA, Alpert C, Dorosh L, Deshmukh SV, Yu H, Klappenbach J, Elwood F, Dinsmore CJ, Fernandez R, Moy L, Young JR.
J Med Chem (2017) 60 : 9676 -9690
PubMed 29156136 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.53 7.41

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4078799 1 7.41
CHEMBL4089118 1 7.32
CHEMBL4102140 1 7.27
CHEMBL4061128 1 7.05
CHEMBL221959 TOFACITINIB 4.0 1 6.97
CHEMBL4089234 1 6.85
CHEMBL4077886 1 6.70
CHEMBL4100288 1 6.68
CHEMBL4066906 1 6.65
CHEMBL4096821 1 6.59
CHEMBL4085737 1 6.58
CHEMBL4071005 1 6.50
CHEMBL4104623 1 6.48
CHEMBL4067915 1 6.38
CHEMBL4072848 1 6.37
CHEMBL4092626 1 6.28
CHEMBL4076473 1 6.22
CHEMBL4066193 ATINVICITINIB 2.0 1 6.22
CHEMBL3703085 1 6.16
CHEMBL4098999 1 6.13
CHEMBL4064171 1 5.92
CHEMBL4079092 1 5.79
CHEMBL4088262 1 5.67

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4078799 IC50 = 39.0 nM 7.41
CHEMBL4089118 IC50 = 48.0 nM 7.32
CHEMBL4102140 IC50 = 54.0 nM 7.27
CHEMBL4061128 IC50 = 89.0 nM 7.05
CHEMBL221959 TOFACITINIB IC50 = 107.0 nM 6.97
CHEMBL4089234 IC50 = 140.0 nM 6.85
CHEMBL4077886 IC50 = 200.0 nM 6.70
CHEMBL4100288 IC50 = 209.0 nM 6.68
CHEMBL4066906 IC50 = 224.0 nM 6.65
CHEMBL4096821 IC50 = 254.0 nM 6.59
CHEMBL4085737 IC50 = 261.0 nM 6.58
CHEMBL4071005 IC50 = 320.0 nM 6.50
CHEMBL4104623 IC50 = 334.0 nM 6.48
CHEMBL4067915 IC50 = 414.0 nM 6.38
CHEMBL4072848 IC50 = 430.0 nM 6.37
CHEMBL4092626 IC50 = 530.0 nM 6.28
CHEMBL4076473 IC50 = 608.0 nM 6.22
CHEMBL4066193 ATINVICITINIB IC50 = 600.0 nM 6.22
CHEMBL3703085 IC50 = 692.0 nM 6.16
CHEMBL4098999 IC50 = 744.0 nM 6.13
CHEMBL4064171 IC50 = 1192.0 nM 5.92
CHEMBL4079092 IC50 = 1635.0 nM 5.79
CHEMBL4088262 IC50 = 2114.0 nM 5.67