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Assay Detail

CHEMBL4033008

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [35S]-MK499 from human ERG expressed in HEK293 cell membranes
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

The Discovery of 3-((4-Chloro-3-methoxyphenyl)amino)-1-((3R,4S)-4-cyanotetrahydro-2H-pyran-3-yl)-1H-pyrazole-4-carboxamide, a Highly Ligand Efficient and Efficacious Janus Kinase 1 Selective Inhibitor with Favorable Pharmacokinetic Properties.
Siu T, Brubaker J, Fuller P, Torres L, Zeng H, Close J, Mampreian DM, Shi F, Liu D, Fradera X, Johnson K, Bays N, Kadic E, He F, Goldenblatt P, Shaffer L, Patel SB, Lesburg CA, Alpert C, Dorosh L, Deshmukh SV, Yu H, Klappenbach J, Elwood F, Dinsmore CJ, Fernandez R, Moy L, Young JR.
J Med Chem (2017) 60 : 9676 -9690
PubMed 29156136 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 4.55 4.91

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3703085 1 4.91
CHEMBL4096821 1 4.88
CHEMBL4102140 1 4.60
CHEMBL3647849 1 4.58
CHEMBL3703025 1 4.50
CHEMBL4077886 1 4.46
CHEMBL4089234 1 4.40
CHEMBL4104623 1 4.40
CHEMBL4061128 1 4.40
CHEMBL4094682 1 4.34
CHEMBL4076821 1 -
CHEMBL4100288 1 -
CHEMBL4078799 1 -
CHEMBL4067915 1 -
CHEMBL4089118 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3703085 IC50 = 12200.0 nM 4.91
CHEMBL4096821 IC50 = 13200.0 nM 4.88
CHEMBL4102140 IC50 = 25000.0 nM 4.60
CHEMBL3647849 IC50 = 26000.0 nM 4.58
CHEMBL3703025 IC50 = 32000.0 nM 4.50
CHEMBL4077886 IC50 = 35000.0 nM 4.46
CHEMBL4089234 IC50 = 40000.0 nM 4.40
CHEMBL4104623 IC50 = 40000.0 nM 4.40
CHEMBL4061128 IC50 = 40000.0 nM 4.40
CHEMBL4094682 IC50 = 46000.0 nM 4.34
CHEMBL4076821 IC50 > 60000.0 nM -
CHEMBL4100288 IC50 > 60000.0 nM -
CHEMBL4078799 IC50 > 60000.0 nM -
CHEMBL4067915 IC50 > 60000.0 nM -
CHEMBL4089118 IC50 > 60000.0 nM -