Assay Detail
Binding
CHEMBL4034228
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Inhibition of recombinant human SphK2 using NBD-sphingosine as substrate after 2 hrs in presence of ATP by HPLC method
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Identification of selective inhibitors of sphingosine kinases 1 and 2 through a structure-activity relationship study of 4-epi-jaspine B.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 4 | 4.54 | 4.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL322333 | DIMETHYLSPINGOSINE | — | 1 | 4.70 |
| CHEMBL1076555 | — | — | 1 | 4.70 |
| CHEMBL2158685 | ABC-294640 | 2.0 | 1 | 4.22 |
| CHEMBL1526855 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL322333 | DIMETHYLSPINGOSINE | IC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL1076555 | — | IC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL2158685 | ABC-294640 | IC50 | = | 60000.0 | nM | 4.22 |
| CHEMBL1526855 | — | IC50 | > | 100000.0 | nM | - |