Assay Detail
Binding
CHEMBL4034741
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of recombinant human full length GST-tagged CDC25A expressed in Escherichia coli BL21-DE3 pLysS using 3-O-methylfluorescein phosphate as substrate after 2 hrs by fluorimetric analysis
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Novel coumarin- and quinolinone-based polycycles as cell division cycle 25-A and -C phosphatases inhibitors induce proliferation arrest and apoptosis in cancer cells.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 19 | 5.57 | 7.26 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL429095 | — | — | 1 | 7.26 |
| CHEMBL4066424 | — | — | 1 | 5.92 |
| CHEMBL4093222 | — | — | 1 | 5.82 |
| CHEMBL4097725 | — | — | 1 | 5.79 |
| CHEMBL4060132 | — | — | 1 | 5.66 |
| CHEMBL4075821 | — | — | 1 | 5.59 |
| CHEMBL4094178 | — | — | 1 | 5.54 |
| CHEMBL4066643 | — | — | 1 | 5.54 |
| CHEMBL4066281 | — | — | 1 | 5.54 |
| CHEMBL4104313 | — | — | 1 | 5.51 |
| CHEMBL4097300 | — | — | 1 | 5.49 |
| CHEMBL4094844 | — | — | 1 | 5.47 |
| CHEMBL4085485 | — | — | 1 | 5.46 |
| CHEMBL4082819 | — | — | 1 | 5.39 |
| CHEMBL4067408 | — | — | 1 | 5.36 |
| CHEMBL4098262 | — | — | 1 | 5.24 |
| CHEMBL4086414 | — | — | 1 | 5.18 |
| CHEMBL4105005 | — | — | 1 | 5.11 |
| CHEMBL4087165 | — | — | 1 | 4.89 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL429095 | — | IC50 | = | 55.0 | nM | 7.26 |
| CHEMBL4066424 | — | IC50 | = | 1200.0 | nM | 5.92 |
| CHEMBL4093222 | — | IC50 | = | 1500.0 | nM | 5.82 |
| CHEMBL4097725 | — | IC50 | = | 1610.0 | nM | 5.79 |
| CHEMBL4060132 | — | IC50 | = | 2190.0 | nM | 5.66 |
| CHEMBL4075821 | — | IC50 | = | 2570.0 | nM | 5.59 |
| CHEMBL4094178 | — | IC50 | = | 2900.0 | nM | 5.54 |
| CHEMBL4066643 | — | IC50 | = | 2880.0 | nM | 5.54 |
| CHEMBL4066281 | — | IC50 | = | 2850.0 | nM | 5.54 |
| CHEMBL4104313 | — | IC50 | = | 3110.0 | nM | 5.51 |
| CHEMBL4097300 | — | IC50 | = | 3230.0 | nM | 5.49 |
| CHEMBL4094844 | — | IC50 | = | 3370.0 | nM | 5.47 |
| CHEMBL4085485 | — | IC50 | = | 3500.0 | nM | 5.46 |
| CHEMBL4082819 | — | IC50 | = | 4040.0 | nM | 5.39 |
| CHEMBL4067408 | — | IC50 | = | 4330.0 | nM | 5.36 |
| CHEMBL4098262 | — | IC50 | = | 5800.0 | nM | 5.24 |
| CHEMBL4086414 | — | IC50 | = | 6600.0 | nM | 5.18 |
| CHEMBL4105005 | — | IC50 | = | 7730.0 | nM | 5.11 |
| CHEMBL4087165 | — | IC50 | = | 13000.0 | nM | 4.89 |