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Assay Detail

CHEMBL4035255

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Functional
Antiplatelet activity in human platelet rich plasma assessed as inhibition of arachidonic acid-induced platelet aggregation preincubated for 2 mins followed by arachidonic acid addition by turbidimetric method relative to control
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Platelet
Confidence 1 — Organism
Curated By Autocuration

Target

Platelet (CHEMBL4296519)
Type CELL-LINE

Publication

Synthesis and mechanistic evaluation of novel N'-benzylidene-carbohydrazide-1H-pyrazolo[3,4-b]pyridine derivatives as non-anionic antiplatelet agents.
Lourenço AL, Salvador RRS, Silva LA, Saito MS, Mello JFR, Cabral LM, Rodrigues CR, Vera MAF, Muri EMF, de Souza AMT, Craik CS, Dias LRS, Castro HC, Sathler PC.
Eur J Med Chem (2017) 135 : 213 -229
PubMed 28453995 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 8 4.99 5.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4065493 1 5.43
CHEMBL4071381 1 5.43
CHEMBL4084418 1 5.43
CHEMBL4062734 1 5.43
CHEMBL4089783 1 5.43
CHEMBL25 ASPIRIN 4.0 1 4.42
CHEMBL11662 OZAGREL 4.0 1 4.28
CHEMBL4099918 1 4.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4065493 IC50 = 3670.0 nM 5.43
CHEMBL4071381 IC50 = 3670.0 nM 5.43
CHEMBL4084418 IC50 = 3670.0 nM 5.43
CHEMBL4062734 IC50 = 3670.0 nM 5.43
CHEMBL4089783 IC50 = 3670.0 nM 5.43
CHEMBL25 ASPIRIN IC50 = 37600.0 nM 4.42
CHEMBL11662 OZAGREL IC50 = 53100.0 nM 4.28
CHEMBL4099918 IC50 = 78900.0 nM 4.10