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Assay Detail

CHEMBL4039214

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Negative allosteric modulation of rat mGlu5 receptor expressed in HEK293A cells assessed as inhibition of glutamate induced-calcium mobilization preincubated for 2.3 mins followed by glutamate addition measured after 1.9 mins by Fluo-4 AM dye based fluorescence assay
44
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Metabotropic glutamate receptor 5 (CHEMBL2564)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Discovery of N-(5-Fluoropyridin-2-yl)-6-methyl-4-(pyrimidin-5-yloxy)picolinamide (VU0424238): A Novel Negative Allosteric Modulator of Metabotropic Glutamate Receptor Subtype 5 Selected for Clinical Evaluation.
Felts AS, Rodriguez AL, Blobaum AL, Morrison RD, Bates BS, Thompson Gray A, Rook JM, Tantawy MN, Byers FW, Chang S, Venable DF, Luscombe VB, Tamagnan GD, Niswender CM, Daniels JS, Jones CK, Conn PJ, Lindsley CW, Emmitte KA.
J Med Chem (2017) 60 : 5072 -5085
PubMed 28530802 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 44 7.65 8.51

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2386849 2 8.51
CHEMBL4077672 2 8.45
CHEMBL4082854 2 8.17
CHEMBL4096818 2 8.14
CHEMBL4076771 2 8.04
CHEMBL2386772 2 8.03
CHEMBL4100830 2 8.02
CHEMBL4093386 2 8.01
CHEMBL2386850 2 7.97
CHEMBL4095685 2 7.96
CHEMBL4062697 2 7.72
CHEMBL4103707 2 7.70
CHEMBL2440659 2 7.62
CHEMBL4084619 2 7.50
CHEMBL2386771 2 7.48
CHEMBL4070410 2 7.32
CHEMBL4090661 2 7.27
CHEMBL4071866 2 7.22
CHEMBL4089038 2 7.01
CHEMBL4076802 2 6.80
CHEMBL3660237 2 6.70
CHEMBL4076952 2 6.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2386849 IC50 = 3.09 nM 8.51
CHEMBL2386849 IC50 = 3.1 nM 8.51
CHEMBL4077672 IC50 = 3.548 nM 8.45
CHEMBL4077672 IC50 = 3.6 nM 8.44
CHEMBL4082854 IC50 = 6.8 nM 8.17
CHEMBL4082854 IC50 = 6.761 nM 8.17
CHEMBL4096818 IC50 = 7.244 nM 8.14
CHEMBL4096818 IC50 = 7.2 nM 8.14
CHEMBL4076771 IC50 = 9.1 nM 8.04
CHEMBL4076771 IC50 = 9.12 nM 8.04
CHEMBL2386772 IC50 = 9.3 nM 8.03
CHEMBL2386772 IC50 = 9.333 nM 8.03
CHEMBL4100830 IC50 = 9.6 nM 8.02
CHEMBL4100830 IC50 = 9.55 nM 8.02
CHEMBL4093386 IC50 = 9.772 nM 8.01
CHEMBL4093386 IC50 = 10.0 nM 8.00
CHEMBL2386850 IC50 = 10.72 nM 7.97
CHEMBL4095685 IC50 = 11.0 nM 7.96
CHEMBL2386850 IC50 = 11.0 nM 7.96
CHEMBL4095685 IC50 = 11.48 nM 7.94
CHEMBL4062697 IC50 = 19.0 nM 7.72
CHEMBL4062697 IC50 = 19.05 nM 7.72
CHEMBL4103707 IC50 = 19.95 nM 7.70
CHEMBL4103707 IC50 = 20.0 nM 7.70
CHEMBL2440659 IC50 = 23.99 nM 7.62
CHEMBL2440659 IC50 = 24.0 nM 7.62
CHEMBL4084619 IC50 = 32.0 nM 7.50
CHEMBL4084619 IC50 = 32.36 nM 7.49
CHEMBL2386771 IC50 = 33.0 nM 7.48
CHEMBL2386771 IC50 = 33.11 nM 7.48
CHEMBL4070410 IC50 = 48.0 nM 7.32
CHEMBL4070410 IC50 = 47.86 nM 7.32
CHEMBL4090661 IC50 = 53.7 nM 7.27
CHEMBL4090661 IC50 = 54.0 nM 7.27
CHEMBL4071866 IC50 = 60.0 nM 7.22
CHEMBL4071866 IC50 = 60.26 nM 7.22
CHEMBL4089038 IC50 = 98.0 nM 7.01
CHEMBL4089038 IC50 = 97.72 nM 7.01
CHEMBL4076802 IC50 = 158.0 nM 6.80
CHEMBL4076802 IC50 = 158.49 nM 6.80
CHEMBL3660237 IC50 = 199.53 nM 6.70
CHEMBL3660237 IC50 = 200.0 nM 6.70
CHEMBL4076952 IC50 = 251.0 nM 6.60
CHEMBL4076952 IC50 = 251.19 nM 6.60