Assay Detail

CHEMBL4040200

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional

Antiviral activity against HBV ayw1 infected in human HepG2(2.2.15) cells assessed as reduction in viral replication by measuring intracellular viral DNA copy number after 6 days byr by real-time qPCR assay

ChEMBL ↗

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Target

Hepatitis B virus (CHEMBL613497) ↗

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Primary target Hepatitis B virus

CHEMBL613497

Anchored from Assay target.

Protein identity Hepatitis B virus

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Hepatitis B virus
Confidence	1 — Organism
Curated By	Autocuration

Target

Hepatitis B virus (CHEMBL613497) ↗

Type	ORGANISM
Organism	Hepatitis B virus

Publication

Expanding the Antiviral Spectrum of 3-Fluoro-2-(phosphonomethoxy)propyl Acyclic Nucleoside Phosphonates: Diamyl Aspartate Amidate Prodrugs.

Luo M, Groaz E, Andrei G, Snoeck R, Kalkeri R, Ptak RG, Hartman T, Buckheit RW, Schols D, De Jonghe S, Herdewijn P.
J Med Chem (2017) 60 : 6220 -6238
PubMed 28682067 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
EC50	3	8.40	9.46
EC90	3	-	-

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL5314362	ENTECAVIR	4.0	2	9.46
CHEMBL141	LAMIVUDINE	4.0	2	8.05
CHEMBL4070180	—	—	2	7.70

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL5314362	ENTECAVIR	EC50	=	0.35	nM	9.46
CHEMBL141	LAMIVUDINE	EC50	=	8.87	nM	8.05
CHEMBL4070180	—	EC50	=	20.0	nM	7.70
CHEMBL4070180	—	EC90	=	6.08	uM	-
CHEMBL141	LAMIVUDINE	EC90	>	2.0	uM	-
CHEMBL5314362	ENTECAVIR	EC90	>	1.0	uM	-