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Assay Detail

CHEMBL4041124

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant Plasmodium falciparum falcipain-2 expressed in Escherichia coli M15(pREP4) using Cbz-Phe-Arg-AMC as substrate by fluorescence assay
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Plasmodium falciparum
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Falcipain 2 (CHEMBL5800)
Type SINGLE PROTEIN
Organism Plasmodium falciparum

Publication

Development of Novel Peptide-Based Michael Acceptors Targeting Rhodesain and Falcipain-2 for the Treatment of Neglected Tropical Diseases (NTDs).
Previti S, Ettari R, Cosconati S, Amendola G, Chouchene K, Wagner A, Hellmich UA, Ulrich K, Krauth-Siegel RL, Wich PR, Schmid I, Schirmeister T, Gut J, Rosenthal PJ, Grasso S, Zappalà M.
J Med Chem (2017) 60 : 6911 -6923
PubMed 28763614 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 5.90 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4096388 1 6.96
CHEMBL374508 E-64 1 6.60
CHEMBL4072275 1 6.60
CHEMBL4083754 1 6.11
CHEMBL4062015 1 6.11
CHEMBL4070637 1 5.88
CHEMBL4078345 1 5.52
CHEMBL2402204 1 5.13
CHEMBL4101714 1 5.02
CHEMBL4100751 1 5.02
CHEMBL4093034 1 -
CHEMBL4082758 1 -
CHEMBL4081250 1 -
CHEMBL4099269 1 -
CHEMBL4090306 1 -
CHEMBL4060936 1 -
CHEMBL4074432 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4096388 IC50 = 110.0 nM 6.96
CHEMBL374508 E-64 IC50 = 250.0 nM 6.60
CHEMBL4072275 IC50 = 250.0 nM 6.60
CHEMBL4083754 IC50 = 780.0 nM 6.11
CHEMBL4062015 IC50 = 780.0 nM 6.11
CHEMBL4070637 IC50 = 1320.0 nM 5.88
CHEMBL4078345 IC50 = 3010.0 nM 5.52
CHEMBL2402204 IC50 = 7330.0 nM 5.13
CHEMBL4101714 IC50 = 9530.0 nM 5.02
CHEMBL4100751 IC50 = 9630.0 nM 5.02
CHEMBL4093034 IC50 > 50000.0 nM -
CHEMBL4082758 IC50 > 50000.0 nM -
CHEMBL4081250 IC50 > 50000.0 nM -
CHEMBL4099269 IC50 > 50000.0 nM -
CHEMBL4090306 IC50 > 50000.0 nM -
CHEMBL4060936 IC50 > 50000.0 nM -
CHEMBL4074432 IC50 > 50000.0 nM -