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Assay Detail

CHEMBL4041386

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at mouse melanocortin receptor 3 expressed in HEK293 cells assessed as inhibition of NDP-MSH-induced cAMP accumulation after 2 hrs by AlphaScreen assay
24
Total Activities
24
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 3 (CHEMBL4774)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Structure-Activity Relationship Studies on a Macrocyclic Agouti-Related Protein (AGRP) Scaffold Reveal Agouti Signaling Protein (ASP) Residue Substitutions Maintain Melanocortin-4 Receptor Antagonist Potency and Result in Inverse Agonist Pharmacology at the Melanocortin-5 Receptor.
Ericson MD, Freeman KT, Schnell SM, Fleming KA, Haskell-Luevano C.
J Med Chem (2017) 60 : 8103 -8114
PubMed 28813605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 24 6.15 6.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4071633 1 6.70
CHEMBL4102048 1 6.52
CHEMBL4104582 1 6.46
CHEMBL4072662 1 6.40
CHEMBL3577981 1 6.30
CHEMBL4075125 1 6.20
CHEMBL4065443 1 6.10
CHEMBL4101128 1 5.90
CHEMBL4095539 1 5.72
CHEMBL4066929 1 5.70
CHEMBL4067947 1 5.70
CHEMBL4093379 1 -
CHEMBL4086679 1 -
CHEMBL4083137 1 -
CHEMBL4066514 1 -
CHEMBL4087835 1 -
CHEMBL4098651 1 -
CHEMBL4072797 1 -
CHEMBL4093505 1 -
CHEMBL4078828 1 -
CHEMBL4096856 1 -
CHEMBL4089149 1 -
CHEMBL4094419 1 -
CHEMBL4077783 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4071633 Kd = 199.53 nM 6.70
CHEMBL4102048 Kd = 302.0 nM 6.52
CHEMBL4104582 Kd = 346.74 nM 6.46
CHEMBL4072662 Kd = 398.11 nM 6.40
CHEMBL3577981 Kd = 501.19 nM 6.30
CHEMBL4075125 Kd = 630.96 nM 6.20
CHEMBL4065443 Kd = 794.33 nM 6.10
CHEMBL4101128 Kd = 1258.93 nM 5.90
CHEMBL4095539 Kd = 1905.46 nM 5.72
CHEMBL4066929 Kd = 1995.26 nM 5.70
CHEMBL4067947 Kd = 1995.26 nM 5.70
CHEMBL4093379 Kd > 3162.28 nM -
CHEMBL4086679 Kd > 3162.28 nM -
CHEMBL4083137 Kd > 3162.28 nM -
CHEMBL4066514 Kd > 3162.28 nM -
CHEMBL4087835 Kd > 3162.28 nM -
CHEMBL4098651 Kd > 3162.28 nM -
CHEMBL4072797 Kd > 3162.28 nM -
CHEMBL4093505 Kd > 3162.28 nM -
CHEMBL4078828 Kd > 3162.28 nM -
CHEMBL4096856 Kd > 3162.28 nM -
CHEMBL4089149 Kd > 3162.28 nM -
CHEMBL4094419 Kd > 3162.28 nM -
CHEMBL4077783 Kd > 3162.28 nM -