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Assay Detail

CHEMBL4041390

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at mouse melanocortin receptor 1 expressed in HEK293 cells assessed as increase in cAMP accumulation after 2 hrs by AlphaScreen assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocyte-stimulating hormone receptor (CHEMBL4077)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Structure-Activity Relationship Studies on a Macrocyclic Agouti-Related Protein (AGRP) Scaffold Reveal Agouti Signaling Protein (ASP) Residue Substitutions Maintain Melanocortin-4 Receptor Antagonist Potency and Result in Inverse Agonist Pharmacology at the Melanocortin-5 Receptor.
Ericson MD, Freeman KT, Schnell SM, Fleming KA, Haskell-Luevano C.
J Med Chem (2017) 60 : 8103 -8114
PubMed 28813605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.98 10.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 10.68
CHEMBL4072797 1 5.58
CHEMBL4098651 1 4.69
CHEMBL4083137 1 -
CHEMBL4077783 1 -
CHEMBL4065443 1 -
CHEMBL4066514 1 -
CHEMBL4075125 1 -
CHEMBL4089149 1 -
CHEMBL4066929 1 -
CHEMBL4086679 1 -
CHEMBL4104582 1 -
CHEMBL4071633 1 -
CHEMBL4093379 1 -
CHEMBL4072662 1 -
CHEMBL4101128 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE EC50 = 0.021 nM 10.68
CHEMBL4072797 EC50 = 2600.0 nM 5.58
CHEMBL4098651 EC50 = 20500.0 nM 4.69
CHEMBL4083137 EC50 > 100000.0 nM -
CHEMBL4077783 EC50 > 100000.0 nM -
CHEMBL4065443 EC50 > 100000.0 nM -
CHEMBL4066514 EC50 > 100000.0 nM -
CHEMBL4075125 EC50 > 100000.0 nM -
CHEMBL4089149 EC50 > 100000.0 nM -
CHEMBL4066929 EC50 > 100000.0 nM -
CHEMBL4086679 EC50 > 100000.0 nM -
CHEMBL4104582 EC50 > 100000.0 nM -
CHEMBL4071633 EC50 > 100000.0 nM -
CHEMBL4093379 EC50 > 100000.0 nM -
CHEMBL4072662 EC50 > 100000.0 nM -
CHEMBL4101128 EC50 > 100000.0 nM -