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Assay Detail

CHEMBL4042014

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Activation of PKC in human platelet assessed as induction of platelet aggregation measured within 15 mins by tribidimetric method
10
Total Activities
5
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 5 — Multiple protein complex / RNA
Curated By Autocuration

Target

Protein kinase C (PKC) (CHEMBL2093867)
Type PROTEIN FAMILY
Organism Homo sapiens

Publication

Isolation of Phorbol Esters from Euphorbia grandicornis and Evaluation of Protein Kinase C- and Human Platelet-Activating Effects of Euphorbiaceae Diterpenes.
Tsai JY, Rédei D, Forgo P, Li Y, Vasas A, Hohmann J, Wu CC.
J Nat Prod (2016) 79 : 2658 -2666
PubMed 27731641 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 5 6.34 8.02
Activity 5 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL279115 PHORBOL MYRISTATE ACETATE 2.0 2 8.02
CHEMBL1863513 INGENOL MEBUTATE 4.0 2 7.14
CHEMBL170518 PROSTRATIN 2 6.04
CHEMBL4071723 2 5.30
CHEMBL4104319 2 5.22

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL279115 PHORBOL MYRISTATE ACETATE EC50 = 9.5 nM 8.02
CHEMBL1863513 INGENOL MEBUTATE EC50 = 72.5 nM 7.14
CHEMBL170518 PROSTRATIN EC50 = 910.0 nM 6.04
CHEMBL4071723 EC50 = 5040.0 nM 5.30
CHEMBL4104319 EC50 = 6080.0 nM 5.22
CHEMBL279115 PHORBOL MYRISTATE ACETATE Activity - -
CHEMBL170518 PROSTRATIN Activity - -
CHEMBL1863513 INGENOL MEBUTATE Activity - -
CHEMBL4071723 Activity - -
CHEMBL4104319 Activity - -