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Assay Detail

CHEMBL4042375

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]alpha-bungarotoxin from rat hippocampus membrane alpha7* nAChR pretreated for 5 mins followed by [125I]alpha-bungarotoxin addition after overnight incubation by gamma counter
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Hippocampus
Subcellular Membrane
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

From pyrrolidinyl-benzodioxane to pyrrolidinyl-pyridodioxanes, or from unselective antagonism to selective partial agonism at α4β2 nicotinic acetylcholine receptor.
Bolchi C, Bavo F, Gotti C, Fumagalli L, Fasoli F, Binda M, Mucchietto V, Sciaccaluga M, Plutino S, Fucile S, Pallavicini M.
Eur J Med Chem (2017) 125 : 1132 -1144
PubMed 27810599 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 10 5.48 7.24

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3603941 1 7.24
CHEMBL3 NICOTINE 4.0 1 6.63
CHEMBL1917232 1 6.37
CHEMBL3603939 1 5.94
CHEMBL4072628 1 4.92
CHEMBL1186005 1 4.84
CHEMBL1186866 1 4.68
CHEMBL4099531 1 4.62
CHEMBL4095303 1 4.05
CHEMBL4074865 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3603941 Ki = 57.0 nM 7.24
CHEMBL3 NICOTINE Ki = 234.0 nM 6.63
CHEMBL1917232 Ki = 430.0 nM 6.37
CHEMBL3603939 Ki = 1150.0 nM 5.94
CHEMBL4072628 Ki = 12000.0 nM 4.92
CHEMBL1186005 Ki = 14600.0 nM 4.84
CHEMBL1186866 Ki = 21000.0 nM 4.68
CHEMBL4099531 Ki = 24000.0 nM 4.62
CHEMBL4095303 Ki = 89000.0 nM 4.05
CHEMBL4074865 Ki > 10000.0 nM -