Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL4042549

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to human GABAA alpha3beta3gamma2 receptor expressed in HEK293 cells assessed as GABA-induced membrane potential change preincubated for 3 mins followed by addition of GABA measured after 3 mins by FLIPR assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-3/beta-3/gamma-2 (CHEMBL2094120)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis and Characterization of a Novel γ-Aminobutyric Acid Type A (GABAA) Receptor Ligand That Combines Outstanding Metabolic Stability, Pharmacokinetics, and Anxiolytic Efficacy.
Poe MM, Methuku KR, Li G, Verma AR, Teske KA, Stafford DC, Arnold LA, Cramer JW, Jones TM, Cerne R, Krambis MJ, Witkin JM, Jambrina E, Rehman S, Ernst M, Cook JM, Schkeryantz JM.
J Med Chem (2016) 59 : 10800 -10806
PubMed 27933953 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 10 6.40 7.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4089504 1 7.96
CHEMBL514974 1 7.54
CHEMBL522055 1 6.84
CHEMBL4090978 1 6.49
CHEMBL2332214 1 6.33
CHEMBL475022 1 6.07
CHEMBL4062225 1 6.03
CHEMBL4071696 1 5.93
CHEMBL4063081 1 5.52
CHEMBL4098713 1 5.29

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4089504 EC50 = 11.0 nM 7.96
CHEMBL514974 EC50 = 29.0 nM 7.54
CHEMBL522055 EC50 = 146.0 nM 6.84
CHEMBL4090978 EC50 = 321.0 nM 6.49
CHEMBL2332214 EC50 = 470.0 nM 6.33
CHEMBL475022 EC50 = 844.0 nM 6.07
CHEMBL4062225 EC50 = 937.0 nM 6.03
CHEMBL4071696 EC50 = 1170.0 nM 5.93
CHEMBL4063081 EC50 = 3020.0 nM 5.52
CHEMBL4098713 EC50 = 5150.0 nM 5.29