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Assay Detail

CHEMBL4042623

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of quorum sensing LasI synthase in Pseudomonas aeruginosa PAO1 harboring reporter plasmid lasB-gfp assessed as reduction in lasB expression measured every 15 mins for 12 hrs by GFP-fluorescence assay
34
Total Activities
30
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Pseudomonas aeruginosa PAO1
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Disulfide Bond-Containing Ajoene Analogues As Novel Quorum Sensing Inhibitors of Pseudomonas aeruginosa.
Fong J, Yuan M, Jakobsen TH, Mortensen KT, Delos Santos MM, Chua SL, Yang L, Tan CH, Nielsen TE, Givskov M.
J Med Chem (2017) 60 : 215 -227
PubMed 27977197 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 26 5.45 6.25
Inhibition 8 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4080882 1 6.25
CHEMBL4066360 1 6.24
CHEMBL4065077 1 6.17
CHEMBL4069062 1 6.14
CHEMBL4084472 1 6.11
CHEMBL4098007 2 6.01
CHEMBL4079124 1 5.95
CHEMBL4105114 1 5.87
CHEMBL4103368 1 5.87
CHEMBL4084326 1 5.78
CHEMBL4075222 2 5.67
CHEMBL4062632 1 5.66
CHEMBL4070229 2 5.64
CHEMBL4077348 1 5.49
CHEMBL4090410 1 5.46
CHEMBL4102421 1 5.45
CHEMBL4094674 1 5.39
CHEMBL2315299 1 5.22
CHEMBL4063268 1 4.92
CHEMBL508112 BENZOTHIAZYL DISULFIDE 1 4.84
CHEMBL122890 AJOENE 1 4.82
CHEMBL4079822 1 4.77
CHEMBL4074170 1 4.68
CHEMBL4067519 1 4.51
CHEMBL241149 2 4.44
CHEMBL2398107 1 4.34
CHEMBL4074916 1 -
CHEMBL4076132 1 -
CHEMBL4080541 1 -
CHEMBL4073101 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4080882 IC50 = 560.0 nM 6.25
CHEMBL4066360 IC50 = 580.0 nM 6.24
CHEMBL4065077 IC50 = 680.0 nM 6.17
CHEMBL4069062 IC50 = 720.0 nM 6.14
CHEMBL4084472 IC50 = 780.0 nM 6.11
CHEMBL4098007 IC50 = 980.0 nM 6.01
CHEMBL4079124 IC50 = 1120.0 nM 5.95
CHEMBL4105114 IC50 = 1340.0 nM 5.87
CHEMBL4103368 IC50 = 1350.0 nM 5.87
CHEMBL4084326 IC50 = 1660.0 nM 5.78
CHEMBL4075222 IC50 = 2140.0 nM 5.67
CHEMBL4062632 IC50 = 2200.0 nM 5.66
CHEMBL4070229 IC50 = 2280.0 nM 5.64
CHEMBL4077348 IC50 = 3250.0 nM 5.49
CHEMBL4090410 IC50 = 3450.0 nM 5.46
CHEMBL4102421 IC50 = 3580.0 nM 5.45
CHEMBL4094674 IC50 = 4080.0 nM 5.39
CHEMBL2315299 IC50 = 6000.0 nM 5.22
CHEMBL4063268 IC50 = 12130.0 nM 4.92
CHEMBL508112 BENZOTHIAZYL DISULFIDE IC50 = 14480.0 nM 4.84
CHEMBL122890 AJOENE IC50 = 15000.0 nM 4.82
CHEMBL4079822 IC50 = 16900.0 nM 4.77
CHEMBL4074170 IC50 = 20920.0 nM 4.68
CHEMBL4067519 IC50 = 31000.0 nM 4.51
CHEMBL241149 IC50 = 36200.0 nM 4.44
CHEMBL2398107 IC50 = 45380.0 nM 4.34
CHEMBL4075222 Inhibition - % -
CHEMBL241149 Inhibition - % -
CHEMBL4098007 Inhibition - % -
CHEMBL4070229 Inhibition - % -
CHEMBL4073101 Inhibition - % -
CHEMBL4080541 Inhibition - % -
CHEMBL4076132 Inhibition - % -
CHEMBL4074916 Inhibition - % -