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Assay Detail

CHEMBL4044718

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of coffee beans alpha-galactosidase pre-incubated for 1 hr followed by p-nitrophenyl-alpha-D-galactopyranoside substrate addition by fluorescence detection based assay
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Coffea arabica
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Alpha-galactosidase (CHEMBL5217)
Type SINGLE PROTEIN
Organism Coffea arabica

Publication

α-Geminal disubstituted pyrrolidine iminosugars and their C-4-fluoro analogues: Synthesis, glycosidase inhibition and molecular docking studies.
Gavale KS, Chavan SR, Kumbhar N, Kawade S, Doshi P, Khan A, Dhavale DD.
Bioorg Med Chem (2017) 25 : 5148 -5159
PubMed 28751199 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.45 7.80
Ki 2 7.66 8.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4073111 2 8.10
CHEMBL4096086 2 7.21

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4073111 Ki = 8.0 nM 8.10
CHEMBL4073111 IC50 = 16.0 nM 7.80
CHEMBL4096086 Ki = 62.0 nM 7.21
CHEMBL4096086 IC50 = 79.0 nM 7.10