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Assay Detail

CHEMBL4046896

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of bovine liver beta-galactosidase pre-incubated for 30 mins with 4-nitrophenyl-beta-D-galactopyranoside substrate before enzyme addition measured over 45 mins at 27 secs intervals by UV/Vis based spectroscopy
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Bos taurus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-galactosidase (CHEMBL3482)
Type SINGLE PROTEIN
Organism Bos taurus

Publication

Picomolar inhibition of β-galactosidase (bovine liver) attributed to loop closure.
Pickens JB, Wang F, Striegler S.
Bioorg Med Chem (2017) 25 : 5194 -5202
PubMed 28844803 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 25 9.12 10.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3359669 1 10.30
CHEMBL3359677 1 10.22
CHEMBL3359672 1 10.15
CHEMBL3359681 1 10.10
CHEMBL3359671 1 9.96
CHEMBL3359666 1 9.96
CHEMBL3359674 1 9.96
CHEMBL3359122 1 9.92
CHEMBL3359668 1 9.89
CHEMBL3359123 1 9.80
CHEMBL3359682 1 9.80
CHEMBL3815031 1 9.74
CHEMBL3359124 1 9.72
CHEMBL3359670 1 9.70
CHEMBL3359667 1 9.64
CHEMBL4091969 1 9.62
CHEMBL3359673 1 9.49
CHEMBL3359678 1 9.49
CHEMBL3359680 1 9.41
CHEMBL3752112 1 9.22
CHEMBL3359679 1 8.63
CHEMBL3753015 1 6.08
CHEMBL3754250 1 5.82
CHEMBL3359683 1 5.70
CHEMBL3359684 1 5.57

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3359669 Ki = 0.05 nM 10.30
CHEMBL3359677 Ki = 0.06 nM 10.22
CHEMBL3359672 Ki = 0.07 nM 10.15
CHEMBL3359681 Ki = 0.08 nM 10.10
CHEMBL3359671 Ki = 0.11 nM 9.96
CHEMBL3359666 Ki = 0.11 nM 9.96
CHEMBL3359674 Ki = 0.11 nM 9.96
CHEMBL3359122 Ki = 0.12 nM 9.92
CHEMBL3359668 Ki = 0.13 nM 9.89
CHEMBL3359123 Ki = 0.16 nM 9.80
CHEMBL3359682 Ki = 0.16 nM 9.80
CHEMBL3815031 Ki = 0.18 nM 9.74
CHEMBL3359124 Ki = 0.19 nM 9.72
CHEMBL3359670 Ki = 0.2 nM 9.70
CHEMBL3359667 Ki = 0.23 nM 9.64
CHEMBL4091969 Ki = 0.24 nM 9.62
CHEMBL3359673 Ki = 0.32 nM 9.49
CHEMBL3359678 Ki = 0.32 nM 9.49
CHEMBL3359680 Ki = 0.39 nM 9.41
CHEMBL3752112 Ki = 0.6 nM 9.22
CHEMBL3359679 Ki = 2.34 nM 8.63
CHEMBL3753015 Ki = 840.0 nM 6.08
CHEMBL3754250 Ki = 1500.0 nM 5.82
CHEMBL3359683 Ki = 2000.0 nM 5.70
CHEMBL3359684 Ki = 2700.0 nM 5.57