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Assay Detail

CHEMBL4047447

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human N-terminal truncated LSD1 (151 to 852 residues) expressed in Escherichia coli using histone H3(1-21)K4(Me1) biotin peptide as substrate measured after 30 mins by TR-FRET assay
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Lysine-specific histone demethylase 1A (CHEMBL6136)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Development and evaluation of 4-(pyrrolidin-3-yl)benzonitrile derivatives as inhibitors of lysine specific demethylase 1.
Mould DP, Bremberg U, Jordan AM, Geitmann M, McGonagle AE, Somervaille TCP, Spencer GJ, Ogilvie DJ.
Bioorg Med Chem Lett (2017) 27 : 4755 -4759
PubMed 28927796 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 5.99 7.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3134377 1 7.39
CHEMBL4071991 1 7.24
CHEMBL4066130 1 6.68
CHEMBL4092760 1 6.58
CHEMBL4087724 1 6.24
CHEMBL4100487 1 6.17
CHEMBL4082476 1 6.09
CHEMBL4105603 1 6.02
CHEMBL4079770 1 5.92
CHEMBL4074738 1 5.58
CHEMBL4079847 1 5.52
CHEMBL4097870 1 5.38
CHEMBL4093703 1 5.36
CHEMBL4101423 1 5.32
CHEMBL4063365 1 5.24
CHEMBL4085006 1 5.05
CHEMBL4066293 1 -
CHEMBL4104027 1 -
CHEMBL4087649 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3134377 IC50 = 41.0 nM 7.39
CHEMBL4071991 IC50 = 57.0 nM 7.24
CHEMBL4066130 IC50 = 210.0 nM 6.68
CHEMBL4092760 IC50 = 260.0 nM 6.58
CHEMBL4087724 IC50 = 570.0 nM 6.24
CHEMBL4100487 IC50 = 680.0 nM 6.17
CHEMBL4082476 IC50 = 820.0 nM 6.09
CHEMBL4105603 IC50 = 950.0 nM 6.02
CHEMBL4079770 IC50 = 1200.0 nM 5.92
CHEMBL4074738 IC50 = 2600.0 nM 5.58
CHEMBL4079847 IC50 = 3000.0 nM 5.52
CHEMBL4097870 IC50 = 4200.0 nM 5.38
CHEMBL4093703 IC50 = 4400.0 nM 5.36
CHEMBL4101423 IC50 = 4800.0 nM 5.32
CHEMBL4063365 IC50 = 5700.0 nM 5.24
CHEMBL4085006 IC50 = 9000.0 nM 5.05
CHEMBL4066293 IC50 > 30000.0 nM -
CHEMBL4104027 IC50 > 30000.0 nM -
CHEMBL4087649 IC50 > 30000.0 nM -