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Assay Detail

CHEMBL4047731

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human histamine H1 receptor expressed in CHO cells assessed as inhibition of histamine-induced intracellular calcium mobilization at 100 nM pretreated for 30 mins followed by histamine addition measured for 1 min by Fluo-4-AM dye based FLIPR method
22
Total Activities
21
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL231)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification of selective 8-(piperidin-4-yloxy)quinoline sulfone and sulfonamide histamine H1 receptor antagonists for use in allergic rhinitis.
Procopiou PA, Ford AJ, Gore PM, Hancock AP, Hodgson ST, Holmes DS, Looker BE, Vile S, Clark KL, Saunders KA, Slack RJ, Watts CJ.
Bioorg Med Chem Lett (2017) 27 : 4914 -4919
PubMed 28958623 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 22 9.10 9.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4060529 1 9.70
CHEMBL4059750 1 9.70
CHEMBL639 AZELASTINE 4.0 1 9.70
CHEMBL4084325 1 9.58
CHEMBL4098917 1 9.46
CHEMBL4102287 1 9.40
CHEMBL4098192 1 9.40
CHEMBL4088036 1 9.27
CHEMBL4069073 1 9.20
CHEMBL4080880 1 9.13
CHEMBL4082646 1 9.08
CHEMBL4088039 1 9.00
CHEMBL4065123 1 9.00
CHEMBL4063163 1 9.00
CHEMBL4080166 1 8.90
CHEMBL4095759 1 8.85
CHEMBL4060765 1 8.70
CHEMBL4068414 2 8.70
CHEMBL4090136 1 8.70
CHEMBL4079248 1 8.68
CHEMBL4085417 1 8.43

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4060529 Kd = 0.1995 nM 9.70
CHEMBL4059750 Kd = 0.1995 nM 9.70
CHEMBL639 AZELASTINE Kd = 0.1995 nM 9.70
CHEMBL4084325 Kd = 0.263 nM 9.58
CHEMBL4098917 Kd = 0.3467 nM 9.46
CHEMBL4102287 Kd = 0.3981 nM 9.40
CHEMBL4098192 Kd = 0.3981 nM 9.40
CHEMBL4088036 Kd = 0.537 nM 9.27
CHEMBL4069073 Kd = 0.631 nM 9.20
CHEMBL4080880 Kd = 0.7413 nM 9.13
CHEMBL4082646 Kd = 0.8318 nM 9.08
CHEMBL4088039 Kd = 1.0 nM 9.00
CHEMBL4063163 Kd = 1.0 nM 9.00
CHEMBL4065123 Kd = 1.0 nM 9.00
CHEMBL4080166 Kd = 1.259 nM 8.90
CHEMBL4095759 Kd = 1.413 nM 8.85
CHEMBL4060765 Kd = 1.995 nM 8.70
CHEMBL4068414 Kd = 1.995 nM 8.70
CHEMBL4090136 Kd = 1.995 nM 8.70
CHEMBL4079248 Kd = 2.089 nM 8.68
CHEMBL4068414 Kd = 2.512 nM 8.60
CHEMBL4085417 Kd = 3.715 nM 8.43