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Assay Detail

CHEMBL4048262

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of human gamma-secretase expressed in HEK293 cells using Notch1-VP16-Gal4 as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Cell Type HEK293
Confidence 0 — Uncurated / Unknown
Curated By Autocuration

Target

Unchecked (CHEMBL612545)
Type UNCHECKED

Publication

Discovery of the First Potent, Selective, and Orally Bioavailable Signal Peptide Peptidase-Like 2a (SPPL2a) Inhibitor Displaying Pronounced Immunomodulatory Effects In Vivo.
Velcicky J, Bodendorf U, Rigollier P, Epple R, Beisner DR, Guerini D, Smith P, Liu B, Feifel R, Wipfli P, Aichholz R, Couttet P, Dix I, Widmer T, Wen B, Brandl T.
J Med Chem (2018) 61 : 865 -880
PubMed 29359565 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 13 6.10 7.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4059711 1 7.77
CHEMBL4075787 1 7.57
CHEMBL4064631 1 6.21
CHEMBL4077007 1 5.73
CHEMBL4069936 1 5.21
CHEMBL4100749 1 5.11
CHEMBL4076827 1 5.09
CHEMBL4077336 1 -
CHEMBL4074838 1 -
CHEMBL4105093 1 -
CHEMBL4086098 1 -
CHEMBL392068 1 -
CHEMBL255473 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4059711 IC50 = 17.0 nM 7.77
CHEMBL4075787 IC50 = 27.0 nM 7.57
CHEMBL4064631 IC50 = 610.0 nM 6.21
CHEMBL4077007 IC50 = 1860.0 nM 5.73
CHEMBL4069936 IC50 = 6100.0 nM 5.21
CHEMBL4100749 IC50 = 7800.0 nM 5.11
CHEMBL4076827 IC50 = 8200.0 nM 5.09
CHEMBL4077336 IC50 > 10000.0 nM -
CHEMBL4074838 IC50 > 10000.0 nM -
CHEMBL4105093 IC50 > 10000.0 nM -
CHEMBL4086098 IC50 > 10000.0 nM -
CHEMBL392068 IC50 < 0.3 nM -
CHEMBL255473 IC50 > 10000.0 nM -