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Assay Detail

CHEMBL4050583

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant rat TRPV4 expressed in CHOK1 cells assessed as inhibition of 4alphaPDD-induced activation pretreated for 5 mins followed by 4alphaPDD stimulation measured for 5 mins by FLIPR assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Pharmacological evaluation of novel (6-aminopyridin-3-yl)(4-(pyridin-2-yl)piperazin-1-yl) methanone derivatives as TRPV4 antagonists for the treatment of pain.
Tsuno N, Yukimasa A, Yoshida O, Suzuki S, Nakai H, Ogawa T, Fujiu M, Takaya K, Nozu A, Yamaguchi H, Matsuda H, Funaki S, Yamanada N, Tanimura M, Nagamatsu D, Asaki T, Horita N, Yamamoto M, Hinata M, Soga M, Imai M, Morioka Y, Kanemasa T, Sakaguchi G, Iso Y.
Bioorg Med Chem (2017) 25 : 2177 -2190
PubMed 28284871 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.49 8.36

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4101768 1 8.36
CHEMBL4099293 1 8.09
CHEMBL3941914 1 8.07
CHEMBL4060956 1 7.66
CHEMBL4077429 1 7.42
CHEMBL4080527 1 7.41
CHEMBL4063752 1 7.33
CHEMBL4083772 1 7.30
CHEMBL4082835 1 7.21
CHEMBL4077790 1 6.75
CHEMBL2133556 1 6.75

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4101768 IC50 = 4.4 nM 8.36
CHEMBL4099293 IC50 = 8.2 nM 8.09
CHEMBL3941914 IC50 = 8.6 nM 8.07
CHEMBL4060956 IC50 = 22.0 nM 7.66
CHEMBL4077429 IC50 = 38.0 nM 7.42
CHEMBL4080527 IC50 = 39.0 nM 7.41
CHEMBL4063752 IC50 = 47.0 nM 7.33
CHEMBL4083772 IC50 = 50.0 nM 7.30
CHEMBL4082835 IC50 = 62.0 nM 7.21
CHEMBL4077790 IC50 = 180.0 nM 6.75
CHEMBL2133556 IC50 = 180.0 nM 6.75