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Assay Detail

CHEMBL4052188

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [125I]-NDP-MSH from mouse MC4R expressed in HEK293 cells after 1 hr by gamma counting
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Melanocortin receptor 4 (CHEMBL3719)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of Mixed Pharmacology Melanocortin-3 Agonists and Melanocortin-4 Receptor Tetrapeptide Antagonist Compounds (TACOs) Based on the Sequence Ac-Xaa1-Arg-(pI)DPhe-Xaa4-NH2.
Doering SR, Freeman KT, Schnell SM, Haslach EM, Dirain M, Debevec G, Geer P, Santos RG, Giulianotti MA, Pinilla C, Appel JR, Speth RC, Houghten RA, Haskell-Luevano C.
J Med Chem (2017) 60 : 4342 -4357
PubMed 28453292 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 7.04 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL441738 AFAMELANOTIDE 4.0 1 8.70
CHEMBL4083717 1 7.89
CHEMBL4078495 1 7.37
CHEMBL4063911 1 7.08
CHEMBL4071063 1 7.03
CHEMBL264190 1 6.92
CHEMBL4079298 1 6.87
CHEMBL4072129 1 6.66
CHEMBL4091894 1 6.56
CHEMBL4102774 1 6.34
CHEMBL4092424 1 6.07

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL441738 AFAMELANOTIDE IC50 = 2.0 nM 8.70
CHEMBL4083717 IC50 = 13.0 nM 7.89
CHEMBL4078495 IC50 = 43.0 nM 7.37
CHEMBL4063911 IC50 = 83.0 nM 7.08
CHEMBL4071063 IC50 = 93.0 nM 7.03
CHEMBL264190 IC50 = 121.0 nM 6.92
CHEMBL4079298 IC50 = 135.0 nM 6.87
CHEMBL4072129 IC50 = 220.0 nM 6.66
CHEMBL4091894 IC50 = 275.0 nM 6.56
CHEMBL4102774 IC50 = 455.0 nM 6.34
CHEMBL4092424 IC50 = 850.0 nM 6.07