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Assay Detail

CHEMBL4053403

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of recombinant human DPP-4 expressed in HEK293 cells using H-Gly-Pro-AMC as substrate measured over 15 mins by fluorescence analysis
17
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dipeptidyl peptidase 4 (CHEMBL284)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Design and Elaboration of a Tractable Tricyclic Scaffold To Synthesize Druglike Inhibitors of Dipeptidyl Peptidase-4 (DPP-4), Antagonists of the C-C Chemokine Receptor Type 5 (CCR5), and Highly Potent and Selective Phosphoinositol-3 Kinase δ (PI3Kδ) Inhibitors.
Schwehm C, Kellam B, Garces AE, Hill SJ, Kindon ND, Bradshaw TD, Li J, Macdonald SJ, Rowedder JE, Stoddart LA, Stocks MJ.
J Med Chem (2017) 60 : 1534 -1554
PubMed 28128944 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 7.25 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL393336 2 7.66
CHEMBL3425747 2 7.56
CHEMBL4063983 2 7.56
CHEMBL4085550 2 7.50
CHEMBL4062977 1 7.36
CHEMBL4071607 1 7.30
CHEMBL4090906 1 7.22
CHEMBL4066703 1 7.16
CHEMBL4077174 1 6.91
CHEMBL4093298 1 6.86
CHEMBL3425748 1 6.84
CHEMBL4098632 1 6.82
CHEMBL4077696 1 6.27

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL393336 IC50 = 22.0 nM 7.66
CHEMBL393336 IC50 = 21.88 nM 7.66
CHEMBL4063983 IC50 = 27.54 nM 7.56
CHEMBL3425747 IC50 = 27.54 nM 7.56
CHEMBL4063983 IC50 = 28.0 nM 7.55
CHEMBL3425747 IC50 = 28.0 nM 7.55
CHEMBL4085550 IC50 = 31.8 nM 7.50
CHEMBL4085550 IC50 = 32.36 nM 7.49
CHEMBL4062977 IC50 = 43.4 nM 7.36
CHEMBL4071607 IC50 = 50.2 nM 7.30
CHEMBL4090906 IC50 = 60.0 nM 7.22
CHEMBL4066703 IC50 = 70.0 nM 7.16
CHEMBL4077174 IC50 = 123.3 nM 6.91
CHEMBL4093298 IC50 = 139.0 nM 6.86
CHEMBL3425748 IC50 = 145.0 nM 6.84
CHEMBL4098632 IC50 = 151.7 nM 6.82
CHEMBL4077696 IC50 = 537.0 nM 6.27