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Assay Detail

CHEMBL4053561

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]N-methylspiperone from human D3 receptor expressed in HEK293 cell membranes after 1 hr by liquid scintillation counting analysis
22
Total Activities
22
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

D(3) dopamine receptor (CHEMBL234)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and Pharmacological Characterization of Novel trans-Cyclopropylmethyl-Linked Bivalent Ligands That Exhibit Selectivity and Allosteric Pharmacology at the Dopamine D3 Receptor (D3R).
Kumar V, Moritz AE, Keck TM, Bonifazi A, Ellenberger MP, Sibley CD, Free RB, Shi L, Lane JR, Sibley DR, Newman AH.
J Med Chem (2017) 60 : 1478 -1494
PubMed 28186762 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 22 7.37 9.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL4077536 1 9.35
CHEMBL4089939 1 9.05
CHEMBL4083903 1 8.82
CHEMBL4068843 1 8.61
CHEMBL2219578 1 8.41
CHEMBL4097669 1 8.01
CHEMBL85606 1 7.96
CHEMBL4079637 1 7.63
CHEMBL4095216 1 7.50
CHEMBL4086613 1 7.46
CHEMBL4105375 1 7.29
CHEMBL4083890 1 6.83
CHEMBL4067792 1 6.59
CHEMBL4099491 1 6.24
CHEMBL4087513 1 5.98
CHEMBL4062165 1 5.96
CHEMBL27162 1 5.82
CHEMBL4076796 1 5.23
CHEMBL4092619 1 -
CHEMBL4064919 1 -
CHEMBL4073520 1 -
CHEMBL4100358 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL4077536 Ki = 0.45 nM 9.35
CHEMBL4089939 Ki = 0.89 nM 9.05
CHEMBL4083903 Ki = 1.51 nM 8.82
CHEMBL4068843 Ki = 2.47 nM 8.61
CHEMBL2219578 Ki = 3.9 nM 8.41
CHEMBL4097669 Ki = 9.81 nM 8.01
CHEMBL85606 Ki = 11.0 nM 7.96
CHEMBL4079637 Ki = 23.4 nM 7.63
CHEMBL4095216 Ki = 32.0 nM 7.50
CHEMBL4086613 Ki = 34.7 nM 7.46
CHEMBL4105375 Ki = 51.7 nM 7.29
CHEMBL4083890 Ki = 149.0 nM 6.83
CHEMBL4067792 Ki = 256.0 nM 6.59
CHEMBL4099491 Ki = 581.0 nM 6.24
CHEMBL4087513 Ki = 1040.0 nM 5.98
CHEMBL4062165 Ki = 1090.0 nM 5.96
CHEMBL27162 Ki = 1520.0 nM 5.82
CHEMBL4076796 Ki = 5890.0 nM 5.23
CHEMBL4092619 Ki > 100000.0 nM -
CHEMBL4064919 Ki > 100000.0 nM -
CHEMBL4073520 Ki > 100000.0 nM -
CHEMBL4100358 Ki > 50000.0 nM -